1.200 ng = 2. 170 μg/ml = μg mg/ml 3. Suppose you're going to run a protein gel, and you need to dilute the running buffer. You have a stock solution that is 20x, and you want to make 500 ml of 1x buffer. How much of the 20x stock solution should you use (in ml)? 4. Suppose you have a DNA solution that is 800 ng/μl, and you want to dilute some of it to make 100 μl of a solution that is 80 ng/μl. How much of your original DNA solution should you use, and how much water should you add? 5. Suppose you have a DNA solution that is 800 ng/μl, and you want to use 0.01 mg of DNA in a reaction. How many microliters of your DNA solution should you use? 6. Suppose you have a sample of plasmid DNA that you want to analyze by performing a restriction digest and a gel. You have 200 μl of your DNA sample, and the DNA concentration is 40 µg/ml. The uncut DNA is 10 kb long, and you expect it to be cut into fragments of 6 kb, 3 kb, and 1 kb. You want to cut enough DNA to give you 50 ng in the 1-kb band, and then load it all on the gel. Show your calculations for everything you would put into the restriction digest tube. The stock concentration of the restriction enzyme is 5 Units/μl, and one unit of enzyme cuts one µg of DNA.

1.200 ng = 2. 170 μg/ml = μg mg/ml 3. Suppose you're going to run a protein gel, and you need to dilute the running buffer. You have a stock solution that is 20x, and you want to make 500 ml of 1x buffer. How much of the 20x stock solution should you use (in ml)? 4. Suppose you have a DNA solution that is 800 ng/μl, and you want to dilute some of it to make 100 μl of a solution that is 80 ng/μl. How much of your original DNA solution should you use, and how much water should you add? 5. Suppose you have a DNA solution that is 800 ng/μl, and you want to use 0.01 mg of DNA in a reaction. How many microliters of your DNA solution should you use? 6. Suppose you have a sample of plasmid DNA that you want to analyze by performing a restriction digest and a gel. You have 200 μl of your DNA sample, and the DNA concentration is 40 µg/ml. The uncut DNA is 10 kb long, and you expect it to be cut into fragments of 6 kb, 3 kb, and 1 kb. You want to cut enough DNA to give you 50 ng in the 1-kb band, and then load it all on the gel. Show your calculations for everything you would put into the restriction digest tube. The stock concentration of the restriction enzyme is 5 Units/μl, and one unit of enzyme cuts one µg of DNA.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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