13 Match each of the QPCR samples (i-v) with the correct amplification plot (A-E) anddetermine the Ct value for each of the amplification curves.Number of template copiesin the QPCR reaction:Amplificationplot (A-E):Ct Value12001000i.1X 106ii. 1X 105iii. 1X 108iv. 1X 107V.NTC (No template control)800600400200AC051015202530Cyclesvi. Create a standard curve graph of template copies vs Ct with the data in the table above.Log of Starting Quantity of DNA vrs C+vii.2624222212018161412כי108642055.566.577.58Log Starting Quantity of DNA (Template copies)If a patient sample has a Ct of 20, interpolate the number of template copies at the start of the PCRreaction using the standard curve you created above?

Quantitative polymerase chain reaction (qPCR) is a widely used molecular biology technique for…

Answered: 13 Match each of the QPCR samples (i-v)…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

Calculate the scan time for a GRE with TR = 30 msec, NEX = 2, Ny = 256, for (a) a single slice and (b) 15 slices.
What are DNA probes Describe three (3) methods that can be used to label DNA probes Describe 2 methods for detection of the hybridized probe List the advantages of using non-radioactively labeled probes vs radioactively labeled probes.
Choose two genes from Figure 4.6 and draw a graph to represent the change in transcription over time.
1. How many TSS sites were identified using this technique? 2. Look at the labels next to each of the annotated TSSs. What are the labels for the TSS sites? 3. What is the coordinate for TSS_tra_16584216?
How can you calculate the final concentrations of these when final volume is 50 µl? • 2 µl of 5 ng DNA template (α2 cDNA in pAWneo2 vector, stock concentration 2.5 ng/µl) • 1 µl of dNTP mix (10 mM dATP, 10 mM dTTP, 10 mM dCTP and 10 mM dGTP) • 2 µl of α2I forward primer (5 µM) • 2 µl of α2I reverse primer (5 µM) • 2.5 µl of OneTaq polymerase (0.5 U/µl)
Nicotinamide Adenine Dinucleotide (NAD) Assay (SIGMA Kit MAK037) analysis of tissue samples initially requires: centrifugation at 13,000g for 10min to homogenise tissue. centrifugation of 20mg of tissue at 2,000rpm with Extraction Buffer. freezing and thawing for 2 cycles of 20min before addition of Extraction Buffer. homogenization of PBS washed tissue with Extraction Buffer.
a. Briefly discuss (using three sentences) how the concepts and/or techniques in molecular biology are applied in COVID-19 pandemic, and: b. For each topic (Covid-19 pandemic), give any issues, benefits, or drawbacks of the application (still using three sentences).
A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This solution is serially diluted 4 times by combining 0.1 mL of each sequential dilution with 9.9 mL of liquid medium. Then 0.1 mL of the final dilution is plated in the plaque assay and yields 21 plaques. What is the initial density of bacteriophages in the original 1 mL? Recall that initial phage density = (plaque number/mL) ×× (dilution factor).
Section Name MAPPING PRACTICE #4 Plasmid pBR 607 is a 2.6 Kb plasmid containing Ampicillin and Tetracycline resistance markers, an origin of replication, and unique restriction sites for the restriction enzymes EcoRI, BamHI, and Pstl. Given the restriction map for pBR 607 for the enzymes EcoRI, BamHI, and Pstl, show on the gel diagram, where the approximate positions of the restriction fragments generated from the restriction digests would be located after carrying out electrophoresis. BamHI 0.2 Kb pBR 607 ECORI 1.94 Kb 0.46 Kb Pstl Size EcoRI EcoRI EcoRI + Standards BamHI + Pstl BamHI Pstl 4.0 Kb 2.2 Kb 2.0 Kb 0.5 Kb
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Considering counting rules, calculate the initial titre of the sample viral stock if the following plaques were counted in a plaque assay. Show clear sample calculations. Table 1: Plaque assay counts for lambda phage stock titre enumeration of average concentration (PFU/mL) Total dilution of viral stock 1/13500000 1/135000000 1/135000000 plated Volume diluted viral stock 0.1 0.1 0.1 plated (mL) PFU replicate 1 TNTC 275 25 Calculated concentration (PFU/mL) replicate 1 PFU replicate 2 TNTC 239 23 Calculated concentration (PFU/mL) replicate 2 Average concentration (PFU/mL)
Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…

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