Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
2. Mitochondria isolated from bovine cardiac muscle, when subjected to sonication, form closed mem- brane vesicles containing the electron transport catalysts and the ATP synthase enzyme located in the inner mitochondrial membrane. A diagrammatic representation of these electron transport particles retaining the capacity for oxidative phosphorylation is given below. NADH- NAD+ tion? H₂O ATP 1/202 NADH Q₁H₂ NAD+ Complex I Complex III ADP Complex IV ADP ATP In the diagram protein complexes colored gray indicate that they are inhibited while complexes colored red, green, etc. are catalytically active. Complex II (a) ( In Panel A indicate which side of the membrane vesicle faced the matrix and which side faced the intermembrane space prior to sonication. During coupled substrate oxidation which compart- ment will be of higher pH and which of lower pH. (b) For the system in Panel A, sonication of mitochondria was carried out in the presence of an excess of ferricytochrome c (i.e., the heme iron is in the ferric state) and Coenzyme Q (CoQ or ubiquinone) enabling both to be trapped into the vesicular cavity upon closing of membrane fragments into spherical vesicles. For oxidation of NADH by O2 coupled to phosphorylation of ADP, in addition to NADH and ADP, what other reagent must be added to the suspension of electron transport particles to observe oxidative phosphorylation and how does this differ from the corresponding process in intact mitochondria?. Why would addition of oligomycin not alter the coupling of oxidation and phosphoryla-

2. Mitochondria isolated from bovine cardiac muscle, when subjected to sonication, form closed mem- brane vesicles containing the electron transport catalysts and the ATP synthase enzyme located in the inner mitochondrial membrane. A diagrammatic representation of these electron transport particles retaining the capacity for oxidative phosphorylation is given below. NADH- NAD+ tion? H₂O ATP 1/202 NADH Q₁H₂ NAD+ Complex I Complex III ADP Complex IV ADP ATP In the diagram protein complexes colored gray indicate that they are inhibited while complexes colored red, green, etc. are catalytically active. Complex II (a) ( In Panel A indicate which side of the membrane vesicle faced the matrix and which side faced the intermembrane space prior to sonication. During coupled substrate oxidation which compart- ment will be of higher pH and which of lower pH. (b) For the system in Panel A, sonication of mitochondria was carried out in the presence of an excess of ferricytochrome c (i.e., the heme iron is in the ferric state) and Coenzyme Q (CoQ or ubiquinone) enabling both to be trapped into the vesicular cavity upon closing of membrane fragments into spherical vesicles. For oxidation of NADH by O2 coupled to phosphorylation of ADP, in addition to NADH and ADP, what other reagent must be added to the suspension of electron transport particles to observe oxidative phosphorylation and how does this differ from the corresponding process in intact mitochondria?. Why would addition of oligomycin not alter the coupling of oxidation and phosphoryla-

Human Physiology: From Cells to Systems (MindTap Course List)
Human Physiology: From Cells to Systems (MindTap Course List)
9th Edition
ISBN:9781285866932
Author:Lauralee Sherwood
Publisher:Lauralee Sherwood
Chapter2: Cell Physiology
Section: Chapter Questions
Problem 10RE: Using the answer code on the right, indicate which form of energy production is being described: 1....
See similar textbooks
icon
Related questions
Question
2. Mitochondria isolated from bovine cardiac muscle, when subjected to sonication, form closed mem- brane vesicles containing the electron transport catalysts and the ATP synthase enzyme located in the inner mitochondrial membrane. A diagrammatic representation of these electron transport particles retaining the capacity for oxidative phosphorylation is given below. NADH NAD+ ( ATP tion? H₂O ADP 1/202 NADH Q₁H₂ NAD+ Q₁ Complex I Complex III ADP Complex IV ATP In the diagram protein complexes colored gray indicate that they are inhibited while complexes colored red, green, etc. are catalytically active. Complex II (a)( In Panel A indicate which side of the membrane vesicle faced the matrix and which side faced the intermembrane space prior to sonication. During coupled substrate oxidation which compart- ment will be of higher pH and which of lower pH. (b) For the system in Panel A, sonication of mitochondria was carried out in the presence of an excess of ferricytochrome c (i.e., the heme iron is in the ferric state) and Coenzyme Q (CoQ or ubiquinone) enabling both to be trapped into the vesicular cavity upon closing of membrane fragments into spherical vesicles. For oxidation of NADH by O2 coupled to phosphorylation of ADP, in addition to NADH and ADP, what other reagent must be added to the suspension of electron transport particles to observe oxidative phosphorylation and how does this differ from the corresponding process in intact mitochondria?. Why would addition of oligomycin not alter the coupling of oxidation and phosphoryla-
Transcribed Image Text:2. Mitochondria isolated from bovine cardiac muscle, when subjected to sonication, form closed mem- brane vesicles containing the electron transport catalysts and the ATP synthase enzyme located in the inner mitochondrial membrane. A diagrammatic representation of these electron transport particles retaining the capacity for oxidative phosphorylation is given below. NADH NAD+ ( ATP tion? H₂O ADP 1/202 NADH Q₁H₂ NAD+ Q₁ Complex I Complex III ADP Complex IV ATP In the diagram protein complexes colored gray indicate that they are inhibited while complexes colored red, green, etc. are catalytically active. Complex II (a)( In Panel A indicate which side of the membrane vesicle faced the matrix and which side faced the intermembrane space prior to sonication. During coupled substrate oxidation which compart- ment will be of higher pH and which of lower pH. (b) For the system in Panel A, sonication of mitochondria was carried out in the presence of an excess of ferricytochrome c (i.e., the heme iron is in the ferric state) and Coenzyme Q (CoQ or ubiquinone) enabling both to be trapped into the vesicular cavity upon closing of membrane fragments into spherical vesicles. For oxidation of NADH by O2 coupled to phosphorylation of ADP, in addition to NADH and ADP, what other reagent must be added to the suspension of electron transport particles to observe oxidative phosphorylation and how does this differ from the corresponding process in intact mitochondria?. Why would addition of oligomycin not alter the coupling of oxidation and phosphoryla-
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 4 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Physiology: From Cells to Systems (MindTap …
Human Physiology: From Cells to Systems (MindTap …
Biology
ISBN:
9781285866932
Author:
Lauralee Sherwood
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Biology: The Dynamic Science (MindTap Course List)
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:
9781305389892
Author:
Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:
Cengage Learning
SEE MORE TEXTBOOKS