3. The laboratory technician dilutes her sample serially 1:10 and plates the following dilutions: 104, 105, and 106. The plates are incubated overnight at 37°C. The colony count is as follows: 10 plate has 359 colonies, 10-5 plate, 36 colonies, and the 106 plate, 3 colonies. What is the total number of cells present in her sample?
Q: After fertilization, the _______________ develop(s) into a fruit and the _______________ develop(s) ...
A: Fertilization in plants (flowering) is called Double fertilization in which there are two sperm cell...
Q: In medicine, there are many essential but difficult topics, phenomena, concepts and problems. The in...
A: Medical science encompasses a wide range of topics that attempt to describe how the human body funct...
Q: What is the natural habitat of E.coli?
A: In this question we will discuss about the natural habitat of E.coli.
Q: Events are triggered by different causes, but all are observed by the disappearance of (1) species i...
A: Mass extinction is the event in which the species corresponding to that geological Era are wiped out...
Q: Red-green colorblindness is an X-linked trait. From which parent can a SON inherit the red-green col...
A:
Q: what natural defenses do you have
A: Studies have shown that there are high levels of antibodies against the given diseases once they get...
Q: What are the possible gametes contributed by the parental's in P1 cross?. ProgenyClass. And how do I...
A: The results can be explained by the alleles of each different gene assorting into gametes independen...
Q: You are growing E. coli in a laboratory in order to study their operons. The growth media you are us...
A: The bacterial gene transcription is regulated by the operon system that regulates the expression of ...
Q: Are there current scientific developments
A: Humans through ages have developed many scientific theories which led to more understanding of our n...
Q: Compare the structure of Gram-positive and Gram-negativebacteria
A: Introduction: Bacteria is a single celled prokaryotic organism. Their cell structure is simpler than...
Q: What are the differences between Bacteria and Viruses?
A: Introduction In this question we will discuss about the differences between Bacteria and Viruses.
Q: The following are photomicrographs of T. spathacea leaf cells in 0.01 M NaCl and 0.30 M NaCl solutio...
A: Introduction: When a cell is in a hypotonic solution it usually swells up because solute conc...
Q: All about the properties of Coronavirus as an Immunogen.
A: Coronavirus also called covid 19 is a novel viral infection which attacks the cells in the lungs and...
Q: Define about X-gal (technically 5-bromo-4-chloro-3-indolyl-b-D- galactopyranoside) ? Explain importa...
A: Introduction In the RDT (recombinant DNA technology), we insert the segment of DNA into the vector ...
Q: Read and highlight ways limiting factors affect the population growth. Examples of how limiting fact...
A: Introduction Limiting factor:- It is anything that constrains a population's size and slows or stops...
Q: How are the organs of plants adopted to perform essential functions?
A: How are the organs of plants adopted to perform essential functions? Introduction: Plants have uniqu...
Q: What are selectable marker genes ? Explain the role of selectable marker gene ?
A: Seletable marker Genes are present in the plasmid and they are help in selection if transformants.Th...
Q: 2. How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other...
A: Note: Ad per Bartleby Guidelines For Remaining Answers Please Repost The Question. Introduction: The...
Q: A bacteriophage λ is found that is able to lysogenize itsE. coli host at 30°C but not at 42°C. What ...
A: Introduction Viruses have two types of life cycles either the Lytic cycle or the Lysogenic life cyc...
Q: Having freckles is determined by a dominant allele (F); no freckles is determined by a recessive all...
A: Genotype is a genetic makeup of an organism which controls the specific trait or group of trait whic...
Q: Why is the Donnan membrane phenomenon important for the homeostasis of the cell
A: Every system has a tendency to attain stability. This system can be abiotic or biotic. To reach at a...
Q: Imagine a human trait that is inherited via genetic maternal effect and found on the X chromosome (x...
A: It is given that the trait is X linked recessive. It means the trait is found when all X chromosomes...
Q: How to calculate the percentage of phage that was filtered using a 0.45 um filter? PFU rep 1- 121 P...
A: Bacteria growing on MF-Millipore filters (thickness, 150 micro m) passed through the underlying memb...
Q: 2. Why is classifying and naming an organism a very important task in biology?
A: Biology is the study of life.And this life is seen within the organisms that live .And classifying a...
Q: Research current trends in the vegan food market "Identify 5 relevant quality sources
A: Food provides our bodies with the necessary energy and nutrients to carry out our everyday tasks. A ...
Q: Explain the major difference between diffusion and osmosis.
A: Osmosis- osmosis is a process by which solvent molecules moves from a region of less to high concent...
Q: Draw a neat, labelled layout for the following OP departments: -Cardiology
A:
Q: In the subject Forensic Photography, In your own words, define the term Photography, Light, Calotype...
A: Forensic photography is mostly used in criminal cases. There are other methods like gene therapy us...
Q: WHAT IS THE ECONOMICS IMPORTANCE OF PENICILLUM DIGITATUM? WITH SOURCE PLEASE.
A: Answer
Q: Earth’s biota were believed to be relative simple, slow-metabolizing life. During what era is this?
A: There is a significant change in the earth's atmosphere and the evolution of animals has made it po...
Q: ure 1 is a bacterial gene (1-180). The first base to be transcribed is the base located at sition 77...
A: Transcription is a process in which RNA is formed with the help of template strand of DNA. It consis...
Q: Get SNR-dependent graphs of BER and interruption probabilities for each state by doing receiver dive...
A: Get SNR-dependent graphs of BER and interruption probabilities for each state by doing receiver dive...
Q: 1. List 3 factors within the problem description that have contributed to the development of dehydra...
A: Note- since we are allowed to do one question at a time, so I can answer 1st question only. for othe...
Q: Please answer, not much defination needed . (1) Parathion causes a very simple inactivation of an en...
A: Parathion It refers to an organophosphate pesticide that has a color of pale yellow to dark brown. I...
Q: Define the method for identifying the DNA pieces ?
A: Gel electrophoresis is a method used for the isolation, identification, and characterization of DNA ...
Q: If a cell with 6 chromosomes undergoes mitosis followed by cytokinesis, how many chromosomes will ea...
A: Mitosis is the process of cell division in which the chromosome replicates and equally distribute in...
Q: nple of a gain-of-function experiment? pholinos to test the function of MyoD e-targeting to test the...
A: Gain of function means when the foreign DNA is inserted in anyone of the concerned cells, the gene e...
Q: Check the pairings that are correct. [mark all correct answers] a. ghrelin; secreted from fat cells ...
A: Ghrelin: Ghrelin is a hormone generated by enteroendocrine cells in the gastrointestinal tract, par...
Q: 1. One individual has blood type AB positive, while another individual has blood type O positive, an...
A: Sickle cell anaemia is an autosome linked recessive trait that can be transmitted from parents to t...
Q: aving freckles is determined by a dominant allele (F); no freckles is determined by a. cessive allel...
A: Gregor Johan Mendel , was an Austrian Monk out Brunn Monastery who studied it Vienna University. He...
Q: Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently photobleac...
A: Introduction: The cell membrane is also called as plasma membrane is a thin semi-permeable membrane ...
Q: Effects of BPA on phosphorylation of MAPK family in RAW264.7 cells conclusion
A: Bisphenol A or BPA is one of the key component of polycarbonate plastics and it causes a wide range ...
Q: 1. You are studying a new gene “X” that you think controls skin color in Bearded Dragons. In order t...
A: Polymerase chain reaction (PCR) It refers to the technique used by biologists to make millions and b...
Q: Humans have ~20,000 genes which create > 100,000 proteins used by our cells. What process enables th...
A: 20,000 genes are translated into more than 100,000 proteins that are used by our own cells for multi...
Q: QUESTION 3 Which of the following is the correct comparision of fertilization in sea urchins and mam...
A: fertilization is the process in which the male gamete meets the female gamete and produces a zygote ...
Q: What are the analogies between the mechanisms controlling the lac operon and those controlling phage...
A: Lac operon λ genetic switches are the ones that are in requirement of the breakdown of lactose. Even...
Q: A person suffering from cystic fibrosis is enrolled in an experimental program where their defective...
A: Introduction: Cystic fibrosis is an autosomal recessive disorder, meaning that is not inherited sole...
Q: , yellow pods are dominant to green pc en a pure yellow plant and a hybrid yell-
A: Alleles- An Individuals receive two variant of each gene from each parent are known as alleles. Thes...
Q: Population A consists of 100 hens that are fully isogenicand that are reared in a uniform environmen...
A: In the field of behavioural genetics, the term "heritability" is used. It is a statistical measure u...
Q: Fungia form domes or discs and detach from the reef when they grow larger. So, they are free to move...
A: 'Coral reefs are one of the oldest and most magnificent forms of living systems on the planet. They ...
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 1 images
- 1. You wish to determine viable counts on a culture of Bacillus subtilis. You begin by pipetting 1 ml of culture into 99 ml of sterile water. After mixing the dilution well you make a series of 4 further dilutions of 10-1 each. From the three most dilute samples, you prepare three pour plates using 1 ml in each. After incubation you find the plate counts of the plates are 16, 245 and 890 respectively. a) Show the dilution scheme. b) What is the estimated viable count (cells/ml) in the original culture? 2. Scientific Notation. Fill in the missing information. a) 4.5 x 109 = _______ b) 50 x 107 = _______ c) 2300 x 1010 = _______ d) 0.54 x 108 = _______Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of amL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further dilutedby successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar.After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gramsample of hamburger?3. A bacterial culture was diluted and results from duplicate plates were obtained as indicated below. What was the number of colony forming units/mL of the original culture? Dilution used for plating 10-2 10-3 104 10-5 10⁰ 10-7 10-8 4. 5. Amount plated 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL Colony counts after incubation (Results from duplicate plates) Too many to count Too many to count 341 413 99 175 27 : 29 7:2 0:0 Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of a mL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further diluted by successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar. After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gram sample of hamburger? Devise a serial dilution scheme to prepare a 10-5 dilution on a plate using the least number of…
- You are working in a medical lab and receive a urine sample from a patient that is displaying urinary tract infection symptoms. You use the urine to set up 8 serial 10-fold dilutions. You decide to spread plate 100 µl of different dilutions. The colony count after incubation overnight: 10-4 dilution has 173 colonies. What is the cfu/ml of this culture?3. You were instructed to add 2.75 mL out of 5.0 mL of an undiluted sample to 125.25 mL of sterile diluent. Instead, you add all 5.0 mL to the 125.25 mL, What was the intended dilution and what was the actual dilution? ited States) E Focus OCT 30 W MacBook P DII DD F7 F8 F9 F10 F4 F5 F6 F3 2$ 4 081 R F G H. K < COAt the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing the following solutions: 0 mM, 300 mM, 600 mM of sucrose. The tubes are randomly labelled A, B, C. 2 mL of each solution were transferred to a different microcentrifuge tube, 20 ul of blood were then added to each tube, and they were mixed well by inverting the tube for multiple times. They were observed and it was determined whether the solution in each tube was hypotonic (Yes or No) relative to the blood cells. In addition, the solutions were diluted with water to help you determine which solution is which sucrose concentration. What sucrose concentration corresponds with each tube? Tube Hypotonic (Yes/No) Hypotonic after 1:1 dilution with water (Yes/No) Most likely Sucrose concentration (mM) A No Yes B Yes Yes C No No
- 1. One (1) mL of spring water were added to a petri dish to which 30 mL of melted Plate Count Agar were added. After incubation, 38 colonies arose on the plate. What was the count of CFUs per mL of the spring water?A 7 year old female patient is admitted to a local hospital for a greenstick radius break. The break is repaired, the patient is sent home and receives a cast a few days later. The patient arrives back at the hospital 20 days afterwards complaining of pain, sweeling under the cast. Upon removal of the cast, there is notable swelling, pus, and a furuncle. a. what type of specimen should you collect? b. What type of instant test would you perform? c. if your clinic ran out of the instant tests, what lower cost method would you use to identify the species? d. What would be the typical course of treatment? e. Due to some cases of antibiotic resistant infections at the same hospital, the doctors want to use a specimen and test for antibiotic resistence, What test would you perform, and which antibiotics should the doctor avoid prescribing?1mL Stock #1 1mL 9mL #2 9mL #3 1mL A 9mL #4 0.1mL 1mL O O B 9mL #5 1mL 1mL 9mL #6 O Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units. 0.1mL 1mk D O
- Refer to the following diagram: 100 ul 100 uL 100 pl 100 µL 100 pl 100 pL 100 pl Volume to transfer A B C D E F G 900 uL in each tube 10 102 10 10 10 10 10 Dilutions E.CoN culture Volume of the diluted sample plated 100 ul 100 μL 100 pL 100 ul Volume of the 10 ml 10 ml original sample plated 10 ml 10 ml Plate D Plate E Plate F Plate G Given that Plate D has too many colonies to count, and Plate G has no colonies at all as a result of serial dilution, draw out all four plates and demonstrate what they might look like (assuming serial dilution has been performed correctly). Based on your drawings, identify the number of CFU/ml for Plate E and Plate F. Show your calculations.1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population ofthe culture, by transferring 25 mlof the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using 1 mlaliquot in 9 mlof 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. EDI Sampling COUNTS 1st dilution 54; 61 TNTC 2nd dilution 3; 7 TNCT TNTC 3rd dilution 0; 0 242; 233 TNTC 4th dilution 0, 0 28: 37 249;246 1st 2nd 3rd TNTC * TNTC = Too numerous to count i. Determine the bacterial count (CFU/ml) every 4 hours of incubation for 12 hours. Show all computations. a. 1s* Sampling b. 2d Sampling c. 3* SamplingFirst There Was One, Then There Were Two RESULTS Results of the plate counts for the samples you worked with. Number of Colonies for Each Dilution Plated Time Sampled 10-3 10-4 10-5 10-6 10-7 10-8 10-9 10:40 TNTC TNTC TNTC 280 TFTC TFTC3 T FIC2 TFTC TFTC 13 TNTC TNTU TNTC TNTC I60 TNTC TNTC 1:00 TFTC q a TFTC O 1:20 11:40 12:00 TNTC TNTC87 TFTC TFTC, TFICg TNTC ITNTC ITNTC TNTC 125 TNTC TNTC TNTC TNTC 164 TFIC 13 T+TC Calculate the cfu/ml for each time sample you worked with in the space below and record those values in the table below.