You wish to determine viable counts on a culture of Bacillus subtilis. You begin by pipetting 1 ml of culture into 99 ml of sterile water. After mixing the dilution well you make a series of 4 further dilutions of 10-1 each. From the three most dilute samples, you prepare three pour plates using 1 ml in each. After incubation you find the plate counts of the plates are 16, 245 and 890 respectively.  a) Show the dilution scheme. b) What is the estimated viable count (cells/ml) in the original culture?   2. Scientific Notation. Fill in the missing information. a) 4.5 x 109 = _______  b) 50 x 107 = _______ c) 2300 x 1010 = _______ d) 0.54 x 108   = _______

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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1. You wish to determine viable counts on a culture of Bacillus subtilis. You begin by pipetting 1 ml of culture into 99 ml of sterile water. After mixing the dilution well you make a series of 4 further dilutions of 10-1 each. From the three most dilute samples, you prepare three pour plates using 1 ml in each. After incubation you find the plate counts of the plates are 16, 245 and 890 respectively. 

a) Show the dilution scheme.

b) What is the estimated viable count (cells/ml) in the original culture?

 

2. Scientific Notation. Fill in the missing information.

a) 4.5 x 109 = _______ 

b) 50 x 107 = _______

c) 2300 x 1010 = _______

d) 0.54 x 108   = _______

 

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