3a)ClustalX software was used to perform multiple sequence alignment of the following five Nco protein sequences designated as Nco1-Nco5 (the provided figure). A pair of degenerate primers was designed to PCR-amplify a DNA segment with the size of approximately 290 bp. With justification, discuss which amino acid sequence blocks would be suitable to design the forward and reverse degenerate primers.
3a)ClustalX software was used to perform multiple sequence alignment of the following five Nco protein sequences designated as Nco1-Nco5 (the provided figure). A pair of degenerate primers was designed to PCR-amplify a DNA segment with the size of approximately 290 bp. With justification, discuss which amino acid sequence blocks would be suitable to design the forward and reverse degenerate primers.
Essentials of Pharmacology for Health Professions
7th Edition
ISBN:9781305441620
Author:WOODROW
Publisher:WOODROW
Chapter9: Administration By The Parenteral Route
Section: Chapter Questions
Problem 9CRQ
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3a)ClustalX software was used to perform multiple sequence alignment of the
following five Nco protein sequences designated as Nco1-Nco5 (the provided figure). A pair of degenerate primers was designed to PCR-amplify a DNA segment with the size of approximately 290 bp. With justification, discuss which amino acid
sequence blocks would be suitable to design the forward and reverse degenerate primers.
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