8. You have sequenced a segment of DNA from a species in the genus Lithobates (true frogs). The sequence is below. 5' GCTTCAGGATGCTCCCATAAA 3' If you make the assumption that this is a template strand for mRNA, and you want to synthesize dsRNA to silence the gene, A. What will the sequence of the sense strand be? Be sure to denote 5' and 3' ends of the transcript. B. What will the sequence of the antisense strand be? Be sure to denote 5' and 3' ends of the transcript. C. What will the fate of the dsRNA be, i.e. which molecular machine will first process it? D. How does this molecular machine process the dsRNA?
8. You have sequenced a segment of DNA from a species in the genus Lithobates (true frogs). The sequence is below. 5' GCTTCAGGATGCTCCCATAAA 3' If you make the assumption that this is a template strand for mRNA, and you want to synthesize dsRNA to silence the gene, A. What will the sequence of the sense strand be? Be sure to denote 5' and 3' ends of the transcript. B. What will the sequence of the antisense strand be? Be sure to denote 5' and 3' ends of the transcript. C. What will the fate of the dsRNA be, i.e. which molecular machine will first process it? D. How does this molecular machine process the dsRNA?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:8. You have sequenced a segment of DNA from a species in the genus Lithobates (true
frogs). The sequence is below.
5' GCTTCAGGATGCTCCCATAAA 3'
If you make the assumption that this is a template strand for mRNA, and you want to
synthesize dsRNA to silence the gene,
A. What will the sequence of the sense strand be? Be sure to denote 5' and 3' ends of the
transcript.
B. What will the sequence of the antisense strand be? Be sure to denote 5' and 3' ends of
the transcript.
C. What will the fate of the dsRNA be, i.e. which molecular machine will first process it?
D. How does this molecular machine process the dsRNA?
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