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- Describe the concept behind sterilization by fi ltration, including examples of applications.With aid of a component layout diagram, describe the principle of operation of little sister sterilizer.MacConkey agar plates represent ________ medium. a differential a minimal a selective both a differential and a selective both a minimal and a selective
- Can culture media be sterilized in the hot air sterilizer? Why or why not?1 H 2 176 old culture Smear too BOTERC Losk like? + b. Failure to apply the decolorizer. + ne c. Failure to apply the safranin. Determine whether bacteria are motle or non-motle by looking at the growth in semi-solid medium What is the concentration of agar in (a) typical solid medium (b) semi sold motility medium? Arawer Q1 on Page 357. 7. Orygen Requirement: + d. Reversal of crystal violet and safranin stains. A questions 1.2 and 3 o Pg 83-84 Refer to the power point Questions 1 Predict the effect of the following "mistakes" made when performing a Gram stain. Your answer needs to address the predicted effect on both Gram-positive and Gram-negative cells. Consider each mistake independently. a. Failure to add the iodine. 414 gait to fer. 2 Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram-positive and Gram-negative cells. This being the case, explain how they stain different cell types in the Gram stain. why? a cell wall. patem in…Define the meaning of Surface sterilization of explants Surface sterilization of explants
- You have a broth medium that you would like to turn into a solid medium in order to do streak plates. What could you add to your media to allow it to solidify? Paragraph В I UCan culture media be sterilized in the hot air sterilizer? Why or why not? (Answer in not more than 5 sentences)Which physical or chemical agent would be the best choice for sterilizing the following items: glass pipettes, tryptic soy broth tubes, nutrient agar, antibiotic solution, interior of a biological safety cabinet, wrapped package of plastic Petri plates? Explain your choices.