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With aid of a component layout diagram, describe the principle of operation of little sister sterilizer.
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- In not more than five lines, outline a method for preparation of tomato cell suspension culture and subculture.1(a) 1. What is the theory upon which intermittent sterilization is based? (B) Is dry or moist heat more efficient as a sterilizing agent? Why? (C) When would it be necessary to use aseptic techniques when inoculating a tube of nutrient broth with dirt? (D)Why do some microorganisms characteristically form a growth of the pellicle type? What environmental factors might alter the formation of a pellicle? (This is not a graded assignment)Describe and give a specific example of a facilitateddiffusion process.
- 1a)A surface sterilisation protocol was carried out to sterilise leaf and stemexplants for evaluating the efficacy of the protocol. All explants were washedthoroughly with distilled water before treating with 50% Clorox for 20minutes. The explants were then rinsed with distilled water before the explants were cultured on plates containing Murashige and Skoog (MS) basal medium. A total of five plates were prepared for each type of explant and each plate consisted of 10 explants. The results recorded after a week are shown in Table. Discuss and conclude the results obtained.Based on the knowledge acquired about the conservation and propagation of microorganisms, propose methodologies for:a) conservation of stock culture (used infrequently) of baker's yeast (S. cerevisiae);b) conservation of the working culture (used daily) of the baker's yeast;c) activation (1st stage of propagation) of the work culture;d) propagation of cultures until inoculating a 100 L reactor toi) production of baker's yeast;ii) ethanol production.Why would the reduction in the viable norovirus be much larger in a phosphate buffer solution than in secondary wastewater effluent for PAA, whereas deactivation reduction would be similar for UV irritation for phosphate buffer and secondary effluent? Briefly explain using concepts you have learned about related to disinfection.
- Describe the appearance of an S. agalactiae colony grown on blood agar. Describe how that colony would differ in appearance from a colony of S. pyogenes.Draw a semi-logarithmic diagram showing the killing kinetics of a vegetative bacterial culture, assuming appropriate temperature, e.g. 100°C. Label the axes. 1.) What are the kinetics for killing bacterial spores at 100°C and 121°C 2.) and what about a mixture of different heat-resistant vegetative cells?N. benthamiana will be infiltrated with a solution containing OD600=0.3 of each experimental Agro containing construct and OD600=0.1 of p19. Calculate the volume of cultures (V construct; V p19) needed according to the formulas: V construct = V final × 0.3/OD600; V p19 = V final × 0.1/OD600. One mL of infiltrate is often enough to complete a small experiment. How to plan your final volume accordingly?
- Explain Ricin is a antineoplastic natural products? please explain at your own words.Discuss the principle of flame sterilization.What is the independent variable(s)? Dependent variable? What is the control? What is the purpose of sanitizing the apple prior to inoculating it with plantarum and L. monocytogenes? Why must this work be done in a biological safety cabinet?