A segment of DNA is isolated and added to a 01 mixture of four deoxynucleotides, two specific oligonucleotide sequences, and heat-stable DNA polymerase. The mixture is then heated to denature the DNA, cooled, and reheated in a number of cycles. Which of the following laboratory techniques does this describe? (A) Enzyme-linked immunosorbent assay (B) Gel electrophoresis (C) Northern blot (D) Polymerase chain reaction (E) Sequencing (F) Southern blot (G) Western blot

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter2: Basic Hematology
Section2.3: Microhematocrit
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A segment of DNA is isolated and
added to a
mixture of four deoxynucleotides,
two specific
oligonucleotide sequences, and
heat-stable
DNA polymerase. The mixture is then
heated
to denature the DNA, cooled, and
reheated
in a number of cycles. Which of the
following
laboratory techniques does this
describe?
(A) Enzyme-linked immunosorbent
assay
(B) Gel electrophoresis
(C) Northern blot
(D) Polymerase chain reaction
(E) Sequencing
(F) Southern blot
(G) Western blot
Transcribed Image Text:A segment of DNA is isolated and added to a mixture of four deoxynucleotides, two specific oligonucleotide sequences, and heat-stable DNA polymerase. The mixture is then heated to denature the DNA, cooled, and reheated in a number of cycles. Which of the following laboratory techniques does this describe? (A) Enzyme-linked immunosorbent assay (B) Gel electrophoresis (C) Northern blot (D) Polymerase chain reaction (E) Sequencing (F) Southern blot (G) Western blot
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