a. STREAK PLATE METHOD: a. Pour melted NA or CWA, following aseptic technique in a petri dish and allow the agar to solidify. b. Sterilize the wire loop and get a loopfull of microorganisms of interest from the petri dish with mix culture done in the last activity. c. Streak very gently over the surface of the agar, taking care not to destroy it, as in figure below. DBS L Figure 1 Figure 2 d. Incubate the dish invertedly and examine the plate after 48 hours. e. Examine your previous petri dish further. Choose another colony and get a loopfull f. This time streak this in an agar slant. g. Incubate and examine tube after 48 hours.

a. STREAK PLATE METHOD: a. Pour melted NA or CWA, following aseptic technique in a petri dish and allow the agar to solidify. b. Sterilize the wire loop and get a loopfull of microorganisms of interest from the petri dish with mix culture done in the last activity. c. Streak very gently over the surface of the agar, taking care not to destroy it, as in figure below. DBS L Figure 1 Figure 2 d. Incubate the dish invertedly and examine the plate after 48 hours. e. Examine your previous petri dish further. Choose another colony and get a loopfull f. This time streak this in an agar slant. g. Incubate and examine tube after 48 hours.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.3: Culture Techniques For Bacteriology

Problem 5RQ

See similar textbooks

Similar questions

In spread plate method, colonies form within the agar and agar surface. In pour plate method, previously prepared agar plates are not required. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.
Which of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.
Neutralization of residual disinfectant could be made by: a. Dilution if the concentration exponent is high b. Dilution if the concentration exponent is low c. Adding specific agent e.g. 3% Tween 80 d. Either a or c e. Either b or c
Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!
In disc diffusion studies, where will the lowest concentration of the tested chemical be in the agar plate? in the agar directly below the disc O in the agar halfway between the disc and the petri dish wall O in the agar furthest away from the disc the cehmical is equally distributed throughout the agar
MEDIA. Match the name of the medium with the physiological test. A medium may be used more than once. Tests may require more than one answer. a. Kligler’s iron agar b. MR-VP broth c. phenol red lactose d. SIM medium e. Simmon’s citrate agar f. skim milk agar g. spirit blue agar h. tryptone broth 2,3-butanediol fermentation carbohydrate fermentation casein hydrolysis citrate utilization hydrogen sulfide production mixed-acid fermentation triglyceride hydrolysis tryptophan degradation
In spread plate method, inoculating loop is used to spread bacteria from the inoculation site. For quadrant streaking, the loop must be flame sterilized between quadrant streaks. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.
The following is a Mannitol Salt Agar plate. What does the yellow color on the right side of this plate indicate? TAW ASM's MicrobeLibrary Ⓒ Reynolds tolerance of the salt mannitol metabolism tolerance of the dyes and chemicals in the agar lactose metabolism
An inoculating loop must be flamed to uniform orange-hot from base to tip A. after culture is transferred to the wire loop but before transfer to new media B. before culture is transferred to the wire loop and after culture is transferred from the wire loop to new media C. after culture is transferred to the wire loop and before culture is transferred from the wire loop to new media D. before culture is transferred to the wire loop
Mueller-Hinton agar needs to be made to specific standards in order to yield reliable, reproducible results. How deep is the agar on a Mueller-Hinton agar plate? a) 2 mm b) 4 mm c) 6 mm d) 8 mm
Describe the microbial growth on the plates of the agar surface swabbed with sample from the surface of your interest (doorknob).
1. What is one advantage of utilizing the pour plate technique over the streak plate technique ? 2. Why must the agar pours be cooled to 45C before use in the pour plate technique? 3. Explain the consequences if a group removed all the agar pours from the water bath at one time and allowed them to sit on the bench for several minutes before using them. 4. Why can the agar pour tubes be rinsed in the sink after the agar is transferred to the Petri plate ? Could you rinse the tubes if the bacteria had been pipetted into the agar pour tubes rather than in the plates? Explain. 5. What would be the result if a student dipped his / her loop in the stock culture during inoculations of each quadrant ? Explain . part B 1. The introduction stated that microbes are mechanically separated or diluted over the surface of the medium . How is this accomplished ? 2. Go to https://commons.wikimedia.org/wiki/File:MacConkey_agar_with_LF_and_LF_colonies . . Which side of the plate (left or right)…