All of the following can achieve sterilization (killing endospores, but not prions) EXCEPT gamma radiation O autoclaving at 121 C for 25 min, 15 psi O x ray radiation O boiling water to 100 C for 30 minutes O dry heat at 180 C for 2-4 hours
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- QUESTION 4 Match the following regarding the Gram stain. A. last step in the procedure primary stain В. crystal violet counterstain C. nigrosin mordant D. 95% ethanol docolorizing agent E. methylene blue F. jodineQuestion 4: Starting with a culture that contains 5.0 x 107 CFU/ml, describe a serial dilution scheme that would give a plate with 50 colonies.QUESTION 5 Regarding the Gram stain procedure: whether Gram-positive or Gram-negative, cells may appear colorless... O A. prior to application of the primary stain OB. prior to application of the counterstain O C. following application of the mordant O D. A. B, and C are all true O E. Only A and B are true O F. Only B and C are true
- Question 2. After sterility test of an injectable it was observed that an injection is failed the test what will you do then depending on the product nature (heat sensitive/ heat resistant) (Not more than one sentence)? Mention the purpose of product inhibition test and how it is performed (Not more than one sentence) ? How will you check the validity of a medium (Not more than one sentence) ?The figure above depicts an agar cube with a side length of 13\, \text{mm}13mm13, start text, m, m, end text. In an experiment, students submerged the cube in red dye for 121212 hours. The red dye permeated 1\, \text{mm}1mm1, start text, m, m, end text on each side, as indicated by the shading in the figure. Volume of a rectangular solid: V = lwhV=lwhV, equals, l, w, h Calculate the volume of the agar cube that remained unpenetrated by the red dye.I U Cardo 12 1三 ニ 三 rI TI3 4. 111 I. 5. What are the basic shapes of bacterial cells? What are the basic Know the terms. Which types of organisms have axial filaments? How would classify the following in terms of gram stain reaction and shape and arrangement of the cells Escherichia coli? Staphylococcus aureus? Streptococcus pyogenes? Bacillus anthracis? Do these organisms cause any important diseases? arrangements? you III
- Question 14 If a suspect bacterial pathogen infecting your patient is known to be "fastidious" which medium would you grow this pathogen on? selective media such as MacConkey agar enrichment medium such as blood agar differential medium such as MSA O all purpose medium such as SAB Question 15יון S All Media Christine 6/7/22, 6:03 PM Question 21 All of the following are types of light microscopes EXCEPT O fluorescent. O scanning tunneling. O phase-contrast. O bright-field. O confocal. > 7 ▬QUESTION 35 At Biosafety Level 2 (BSL-2): There are no risks O Work is done with deadly, incurable pathogens O A lab coat is not needed O There is moderate risk and a lab coat and gloves are required QUESTION 36 Sterilization is: Gentle heating to kill some microbes O Killing all microbes in a given area O Used in aseptic technique O b and c QUESTION 37 Click Save and Submit to save and submit. Click Save All Answers to save all answers. P Type here to search 立
- In order to do electron microscopy the samples had to be specially prepared. Were the cells alive at the time of viewing? Explain why you said yes or no I need help answering this queshtion the answer is with the article and it has to be as short as possible URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/Why would the following medium not be considereda chemically defined medium: glucose, 5 grams (g);NH4Cl, 1 g; KH2PO4, 1 g; MgSO4, 0.3 g; yeast extract, 5 g;distilled water, 1 liter? What is aseptic technique andwhy is it necessary?A culture of S. cerevisea has an overnight OD of 2.3 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies. How much of the 2.3 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ul