Answer all the questions. 1. How do you calculate total magnification? 2. Which lens requires the use of cedarwood oil? Why is it important to use oil when focusing under this lens?
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Answer all the questions.
1. How do you calculate total magnification?
2. Which lens requires the use of cedarwood oil? Why is it important to use oil when focusing under this lens?
Step by step
Solved in 2 steps
- Compute the total magnification. [Use 10x magnification for eyepiece]1. Scanning2. LPO3. HPO4. OIOWhat is the DFV of a 40x objective lens if the DFV of a 10x objective lens is 3 mm? Express your answer in μ (micra)Answer these questions by watching the YouTube videos and reviewing the Powerpoints from Lab #4. 1. What is Refraction of light? 2. What is the difference between the Ocular lens and the Objective lens? 3. What is the purpose of the Revolving Nosepiece? 4. What is the difference between the Course Adjustment Knob and the Fine Adjustment Knob? 5. How do you calculate Total Magnification? 6. What is Resolution in terms of Microscopy? 7. What is the purpose of Oil Immersion? 8. What is the Diffraction Barrier and why does it exist? 9. What is the purpose of using stains and fluorescent dyes in microscopy? 10. What is the advantage of using an Electron Microscope? 11. What Objective lens should you always start with? 12. What is the purpose of the Iris Diaphragm on the Condenser? 13. How do you know your Objective lens has been adjusted properly? 14. Why should you not use Kimwipes…
- 1. Calculate the total magnification for the following: A. Scanning power objective = 5x; eyepiece =4x B. High power objective = 40x; eyepiece= 5x c. Low power objective = 10x; eyepiece= 10xTotal Magnification is obtained by Select one: A. Magnifying power of both the objective lens and eyepiece B. Magnifying power of eyepiece C. Magnifying power of condenser lens D. Magnifying power of the objective lens1. What total magnification will be achieved if the 10× eyepiece and the 10× objective are used?___________
- 1. In the following table, fill in the missing information in order to calculate the Total Magnifica- tion for each objective lens. Use the information provided in the laboratory exercise to help you. Magnification of Magnification of Objective Lens Total Magnification Objective Lens Ocular Lens Lowest Power 4X Higher Power 10X Greater Power 40X Highest Power 100X 2. What would the total magnification be if a microscope had 15X ocular lenses and 4X, 10x, 45X and 95X objective lenses? Insert the correct information in the following table. Magnification of Objective Lens Magnification of Objective Lens Total Magnification Ocular Lens 4X Objective lens 10X Objective lens 45X Objective lens 95X Objective Lens5)The simulation asks you about the TOTAL magnification of when you use the 40x objective. This total magnification is based on the the amount of magnification provided by the ocular lens in the eyepiece, as well as the magnification of the objective. Based on the answer given in the simulation, what is the magnification of the ocular lens? (enter the number only) Answer valueIn this image a stage micrometer and an ocular micrometer are aligned so that the 10 o.u. line aligns with the 100um line. The 200um line of the stage micrometer overlaps with the 90 o.u. line of the ocular micrometer. What is the calibration for this lens? eyepiece graticule 40 50 60 70 s0 9 100 stage micrometer 0.8 um/ou O 8 um/ou O 1.25 um/ou 0.25 um/ou
- 8.Explain what the coarse focus knob does and what the fine focus knob does. 9. What is different between the 100X objective and the other objective lenses? In other words, what does the 100X need in order to use it? please give answer for both pleaseWhich of the following is NOT used to adjust the intensity of light? Question 1 options: iris diaphragm rheostat base diaphragm revolving nosepieceWhich lens requires the use of cedarwood oil? Why is it important to use oil when focusing under this lens?