arbo 1. Explain what deoxyribonuclease (DNase) does. ation . Ex Do you think this enzyme exists in humans? Justify your answer. 2. What are the main components (building blocks)of a nucleotide? cid i RET s. B 3. Define depolymerization. esu 4. What are the bonds that are broken during the chemical reaction that results from the activity of DNase?

arbo 1. Explain what deoxyribonuclease (DNase) does. ation . Ex Do you think this enzyme exists in humans? Justify your answer. 2. What are the main components (building blocks)of a nucleotide? cid i RET s. B 3. Define depolymerization. esu 4. What are the bonds that are broken during the chemical reaction that results from the activity of DNase?

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 1P: Semiconservative or Conservative DNA Replication If 15N-Iabeled E. coli DNA has a density of 1.724...

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The Basic Structure of DNA 1. Nucleotides are the monomer subunits of DNA polymers. Review the structure of a nucleotide by labeling the three main parts: the sugar, the phosphate group and the nitrogenous base of the one shown below. NH2 N- || ОН 2. Which part (sugar, phosphate, or nitrogenous base) of the four types of nucleotides differ? 3. Based on the complementary base pairing rules we know that: A(denosine) pairs with and that G(uanine) pairs with 4. What two types of bonds are found in a DNA molecule and where are they found? а. bonds are found b. bonds are found O=a-o
May you please help me with this? A sample of purified DNA was incubated with deoxyribonuclease (DNAse) at 37oC. An aliquot was removed from the reaction mixture every minute for 5 minutes and the A260 recorded. The following data were obtained. Time (min) A260 0 0.60 1 0.64 2 0.67 3 0.70 4 0.72 5 0.73 Describe the action of deoxyribonuclease on DNA and explain the increase in A260.
True or False 1. Histone proteins are able to associate with DNA segments because of the anionic nature of the amino acids arg and lys. 2. DNA molecules can perform their function in replication and transcription as long as the hydrogen bonds between the bases remain intact. 3. A double helix makes one complete turn every 10 residues (3.4nm). Therefore the distance between base pairs is 0.34nm.
protein and why? 6. Shown here is a hybrid of DNA and RNA. Label which one is which. What do the blobs represent? 7. A mad scientist has all the molecules necessarv for nrotein cynthoni
Energy that drives the attachment of a nucleotide to the end of a growing strand of DNA comes from _____. a. the nucleotide b. phosphate-group transfers from ATP c. DNA polymerase
Helicase Unwinding of the E. coli Chromosome Hexameric helicases, such as DnaB, the MCM proteins, and papilloma virus El helicase (illustrated in Figures 16.22 to 16.25), unwind DNA by passing one strand of the DNA duplex through the central pore, using a mechanism based on ATP-dependent binding interactions with the bases of that strand. The genome of E. coli K12 consists of 4,686,137 nucleotides. Assuming that DnaB functions like papilloma virus El helicase, from the information given in Chapter 16 on ATP-coupled DNA unwinding, calculate how many molecules of ATP would be needed to completely unwind the E. coli K 12 chromosome.
Semiconservative or Conservative DNA Replication If 15N-Iabeled E. coli DNA has a density of 1.724 g/mL, 14N-labeled DNA has a density of 1.710 g/mL, and E. coli cells grown for many generations on 14NH4+as a nitrogen source are transferred to media containing 15NH4+as the sole N-source, (a) What will be the density of the DNA after one generation, assuming replication is semiconservative? (b) Suppose replication took place by a conservative mechanism in which the parental strands remained together and the two progeny strands were paired. Design an experiment that could distinguish between semiconservative and conservative modes of replication.
TRUE OR FALSE 1. The RNA molecules could be found in the nucleoplasm 2. The cyclobutane pyrimidine dimers are associated with CG to AT mutations 3. The nucleotides are joined by phosphodiester bonds 4. The mRNA carries the amino acids for elongation in protein synthesis 5. The DNA is present in the mitochondria 6. A blue coloration in Bial’s test indicates the presence of pentoses 7. Sodium hydroxide is the hydrolyzing agent in ammonium molybdate test 8. A black precipitate forms when a purine reacts with silver ions 9. Hydrolysis is the breaking of a bond in a molecule using water 10. The phosphate backbone is the site of cleavage during the DNA hydrolysis
53A%252F%252FIMS.mheducation.com%252Fmghmic signment Saved 2 attempts left Check my work Enter your answer in the provided box. Given two complementary strands of DNA containing 1.08 x 102 base pairs each, calculate the ratio of two separate strands to hydrogen-bonded double helix in solution at 3.32 x 102 K. (Hint: The formula for calculating this ratio is e AE/R", where AE is the energy difference between hydrogen-bonded double- strand DNAS and single-strand DNAS and R is the gas constant.) Assume the energy of hydrogen bonds per base pair to be 7.70 x 10 kJ/mol under laboratory conditions. This model is a simplification of reality. Guide
HN, NH. NH H2N° А. В. Consider the 3 structures shown. Which of these is (normally) NOT present in either DNA or RNA? (select all that apply) А В none of the above ZI C.
TRUE or FALSE. If false, write the word/s that make(s) the statement incorrect. 1.The 2 subunits of DNA PoI II are called clamp loader and sliding clamps. 2. In eukaryotes, replication and transcription occur in the nucleus, while translation occurs in the cytoplasm.
are key p of this bou. and lucleohole Nucleic Acids genetic infromating 2. RAlA 2. 4. Enzymes control the rates at which the chemical reactions in a cell take place. A. Define the following terms: 1. catalyst: 2. enzyme: 3. substrate: 4. active site: 5. energy of activation: B. Use all of the above terms to describe how enzymes work in a few sentences: