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- A culture consisting of 100 litres of nutrient medium containing 12 g l-1 of growth limiting substrate is inoculated with 10g of bacteria in a batch fermenter. The yield of biomass from substrate is 0.05 g g-1 . The maintenance coefficient is 2.0 g g-1 h -1 . The specific rate of product formation due to maintenance is 1.0 h-1 . The maximum specific growth rate of the organism is 0.3 h -1 . YPX= 7.7 and product formation is directly linked with metabolism. a) What is the time required to produce 15 g of biomass? !!!!! ANS IS 3.07 HRS!!!! STEPS on how to reach this pleaseMacConkey agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. Define what type(s) of media MacConkey agar is, using as many terms in the answer list as are applicable.Describe how a Triple Sugar Iron agar tube would appear if the following situation was true. Please note that all items in the following list are occurring in the same tube of agar. 1. Positive for glucose fermentation 2. Negative for lactose and sucrose fermentation 3. Positive for H2S production 4. Negative for gas production
- Describe characteristics of Proteus Vulgaris in the Agar: (How does colonies look like (color) and explain does it grow on that agar. Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient AgarIf a yellow halo is present around a colony on a mannitol salt agar (MSA) plate, the bacterium cannot ferment mannitol.Why does motility media contain a lower amount of agar than ordinary agar?
- MacConkey Agar Lactose fermenter or non-fermenter: ___________________________ Gram reaction based on MAC Growth: ___________________________ Coliform or non-coliform: _____________________________________In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with moldWhat is the unknownn organism based on this biochemical test results? Gram Negative Oxidase Positive Catalase Positive MAC: Lactose Fermenter MSA: Mannitol Fermenter Gelatin Hydrolysis: Negative Indole Production: Negative Motility Indole Ornithine Medium: Negative MR/VP Test: Negative Nitrate Test: Negative Simmon Citrate Test: Positive Urease Test: Positive Possible Organisms: Vibrio fischeri Enterococcus faecalis Micrococcus luteus Streptococcus pneumoniae Pseudomonas aeruginosa Proteus mirabilis Acinetobacter baumannii Staphylococcus aureus Mycoplasma pneumoniae Escherichia coli Candida albicans Salmonella enterica Listeria monocytogenes
- Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind of media based on what kind of microorganisms it allows to growDraw two tubes showing how you have inoculated microorganisms in urease and in nutrient agarMSA (Mannitol Salt Agar) Match the description to the correct Bacterium A= growth, agar is yellow B= Growth, agar is not yellow C= no growth