Brownian movement of bacteria: O a Can be observed by the of soft agar technique O b. Can be observed by staining of Staphylococcus aureus Oc. Is common in Escherichia coli or Proteus vulgaris O d. Is common in Staphylococcus aureus e. Can be observed at the edge of a microscope
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Bacterial Morphology
The bacteria are prokaryotic organisms that are single-celled, and are found to exist as free-living and possess a microscopic size. The morphology is found to vary in the bacteria, where some of them are identified as individual organisms and the others are detected as colonies. The size and shape of the bacterial cell also represent its morphology.
Bacterial cell structure
Bacteria are single-celled, tiny creatures that may enter healthy tissues and grow rapidly. Bacteria are microscopic organisms that are tiny and unicellular. These are members of the prokaryote kingdom. They live in water, air, soil, and all-natural environments. They are used in industrial and therapeutic processes, and they support a wide range of plant and animal life. The first organism to appear on the planet. Bacteria-like creatures are the oldest known fossils. Bacteria can consume a wide range of organic and inorganic elements, and some may even survive in harsh conditions.
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- A. Microscopic Observation of Cells Wet Mounts, Gram stain and spore stain 1 Describe the microscopic morphology and behavior of each species observed with a wet mount (A. serpens and E. gracilis). 2 Describe the microscopic morphology of each sample observed with a Gram stain (S. aureus, P. aeruginosa, B. subtilis and dental plaque). Indicate the Gram reaction of each. 3Describe the microscopic morphology of each sample observed with the spore stain (P. aeruginosa and B. subtilis). In which of the sample can you see spores?I. Match the bacterium being described in column X with its best classification found in column Y. Choose the best answer. Column X1. Staphylococcus aureus can be grown on mannitol salt agar that contains 7.5% NaCl. The bacterium is________. 2. Lactobacillus acidophilus grows best at pH 4.5. It is considered a(n) ________. Column Y A. acidophile B. alkalophile C. barophile 3. A bacterium that thrives in the Great Salt Lake but not in fresh water is probably a ________.4. Bacteria isolated from the bottom of the ocean need high atmospheric pressures to survive. They are________.5. A bacterium that thrives in a soda lake where the average pH is 10.5 can be classified as a(n) ________. D. halotolerant E. halophileWhich can infect hur Table 1: Absorbance and appearance of broth tubes at various temperatures. E. colin B. stearothermophilus Temperature°C Absorbance Visual Absorbance Visual 4 0.04 Clear 0.06 Clear tre Light turbid Very turbid Almost clear 25 0.66 0.09 Clear Light turbid Very turbid 37 1.48 0.55 which 55 0.25 1.29 oledzethe mes he alhe ive to edeut et brahell bras atnstooinieib o ho leviviue zwolls ti saun
- A Gram stain result from cerebrospinal fluid (CSF) indicates Gram positive cocci (G+C) in pairs. Which of the following would be the presumptive identification: O Haemophilus influenzae O None of the answers apply O Streptococcus pneumoniae O Streptococcus pyogenes O Neisseria meningitidis O E. coli O Staphylococcus aureus1. The colonies on a negative MSA plate would appear _____________. 2. "E. Coli and S. epidermidis were chosen to represent Gram-negative and Gram-positive bacteria, respectively. For a given antibiotic, is there a difference in susceptibility between the Gram-positive and Gram-negative bacteria?" "No, they are usually similar" "yes, drugs that target the cell membrane are more effective on gram negatives" "yes, drugs that target the cell wall are more effective on gram negatives" "yes, drugs that target the cell wall are more effective on gram positives" how can I tell the differenceIsolate #2: Cell Morphology (shape) а. b. Isolate #2: Gram stain result Gram Postive. (The color I see on my screen is purple.) c. List at least two genera (not species) of bacteria that are consistent with the Gram reaction and morphology you observed. d. Are any of these genera are normal flora on the skin? If so which one(s)? ***Please answer the four questions base on the picture provided.***
- D Question 37 Based on the data below, how much more UV resistant was B. licheniformis relative to S. aureus? (All controls had growth.) - covered with lid Organisms Exposure 20 sec Staphylococcus aureus 5 sec + 20 sec Bacillus licheniformis + 10 sec 80 sec times to UV light 40 sec 2.5 min 160sec 5 min 10 min 5 min 20 min 10 min 40 min 10 min + 40 minAnswer the following questions: 1. Define a bacterial colony. 2. What is the difference between macroscopic and microscopic appearance of bacteria? 3. State the three standard terms used to describe single colonies on agar plates. 4. State and define the three types of growth that may be seen in a broth culture. 5. State three basic shapes of bacteria. 6. State and describe the different arrangements of cocci. 7. What is the difference between true motility and Brownian motion?1. If you expose E.coli to UV light at 30 seconds, 1 minute, 3 minutes and 5 minutes, what are your expected results? 2. If you expose. B. cereus to UV light at 30 seconds, 1 minute, 3 minutes and 5 minutes, what are your expected results? 3. If you do not remove the cover of the plate before exposure to UV light at 5 minutes for E.coli and B. cereus, what are your expected results? Help with 1,2 & 3 please
- Pregunta 7 Diluting a culture of bacteria to detemine the cancentration OC) Generally not required for well-established E. ull cultures O (D) Both A and B are correct O A) Performed on a liquid bacterial culture to determine the type of organism present O (B) Involves the use of spread plating Pregunta 8 Preparing a cell extract O (B) May involve the use of a detergent O (D) A-C are correct OWs done to prepare a sample for chromatography O (C) May involve the use of an enzyme Pregunta 9 Preparing a cell extract as we did in class O A Resulted in a cell extract with all denatured proteins pok O (C) Both B and D are correct O (B) Involved SDS orm oring O (D) Resulted in a cell extract that maintalned protein tertiary structures TeamsMedically Significant Bacteria: Causative Agent and Disease Profile ITEM IMPORTANCE OF BACTERIA PROFILE BACTERIAL PROFILE I MICROORGANISM/CAUSATIVE AGENT A GRAM REACTION B OXYGEN REQUIREMENT C SIZE D SHAPE E HABITAT F DISCOVERY G MICROSCOPIC IMAGE II DISEASE PROFILE A DISEASE/S B SYMPTOMS OF THE DISEASE C INCUBATION PERIOD D MODE OF TRANSMISSION E DIAGNOSIS F TREATMENT G PREVENTION H NO OF DAYS BEING SYMPTOMATIC I IMAGE OF INFECTED PATIENT GENERAL INSTRUCTIONS: Using the template provided above, fill out the information for a bacteria. YOU CAN CHOOSE ANY BACTERIA from 1-60 BELOW: List of medically significant bacteria Aerobic Gram (+) Cocci S. epidermidis S. saprophyticus Streptococcus pyogenes Streptococcus agalactiae Enterococcus(E. faecalis, E.…From a contaminated intravenous catheter you isolate Gram-positive cocci, beta-hemolytic, and catalase positive. MSA results below (grown aerobically): Which pathogen from the list below have you isolated? O Streptococcus pyogenes_ O Staphylococcus aureus_ Staphylococcus epidermidis_ Streptococcus pneumoniae_