C. b. For each of the 5 STR loci, draw the expected pattern of bands on the gel below. Show the alleles for the seized tusk and the elephant you identified as a potential match. Also, include a DNA ladder that has DNA fragments that are75, 125, 150, and 175 base pairs (bp) in size. Base Pairs 200 180 160 140 120 DNA Ladder Seized Tusk Matching Elephant --- dsdor Elephant 1 Elephant 2 Elephant 3 Elephant 4 Elephant 5 Elephant 6 Elephant 7 Elephant 8 Elephant 9 Elephant 10 FH71 62 64 62 62 62 64 62 64 62 64 64 64 60 62 62 62 62 62 62 FH19 FH60 FH67 97 189 147 193 147 97 185 147 95 193 147 103 183 147 95 185 147 97 193 147 91 193 147 105 187 145 95 187 147 95 187 145 91 193 147 97 193 149 97 193 151 97 193 147 97 193 147 103 193 147 97 193 147 97 189 147 95 193 147 105 62 FH129 152 154 152 152 152 162 152 152 152 160 152 160 156 160 152 160 160 160 152 156 T Table 2: Genotypes using five STR loci for 10 slaughtered elephants. How does the pattern of bands in the gel support your conclusion about which elephant was the source of the seized tusks?

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Chapter10: Biotechnology
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Please solve question b) and c) * The seized tusks came for elephant #8.
1) Pretend you are investigating a newly seized tusk and wonder if the tusk is from a recently discovered crime
where 10 elephants from the same population were found dead with their tusks removed. The genotype of the
seized tusk for five short tandem repeat (STR) loci is shown in Table 1. Allele names for each of the STR loci
correspond to their size in base pairs (bp). Table 2 lists the genotypes of the same five STR loci for 10 recently
slaughtered elephants.
C.
Did the seized tusk come from one of the recently
slaughtered elephants? If so, which one, or if no,
why not? The seized tusks came from
elephant #8. This is because the seized
elephants matches Perfectly with the decreased
PCR. Since everyone has DNA that is unique,
it was an obvious match-
a.
b. For each of the 5 STR loci, draw the expected
pattern of bands on the gel below. Show the alleles
for the seized tusk and the elephant you identified
as a potential match. Also, include a DNA ladder
that has DNA fragments that are 75, 125, 150, and
175 base pairs (bp) in size.
Base
Pairs
200
180
160
140
120
DNA
Ladder
CHR
Seized
Tusk
Matching
Elephant
vollidsdongs 201
Seized Tusk FH19 FH60
Allele 1:
Allele 2:
Elephant 1
T
able 1: Genotype for five STR loci recovered from a
seized elephant tusk.
Elephant 2
Elephant 3
Elephant 4
Elephant 5
Elephant 6
Elephant 7
FH67 FH71
193 147 97 62
193 147 103 62
Elephant 8
Elephant 9
Elephant 10
FH19
FH67 FH71
189
62
193
64
185
62
193
62
183
62
185 147
64
193
147
62
193
64
187
62
187
64
187
64
193
64
193
60
193
62
193
62
193 147
103
62
193 147
97
62
193
147
97
62
189
147
95
62
193 147 105 62
FH60
147 97
147 97
147
95
147
147 95
97
91
147
105
145
95
147 95
145
91
147 97
149 97
151
97
147 97
FH129
152
160
103
FH129
152
154
152
152
152
162
152
152
152
160
152
160
156
160
152
160
160
160
152
156
T
Table 2: Genotypes using five STR loci for 10 slaughtered
elephants.
How does the pattern of bands in the gel support your conclusion about which elephant was the source of the seized
tusks?
Transcribed Image Text:1) Pretend you are investigating a newly seized tusk and wonder if the tusk is from a recently discovered crime where 10 elephants from the same population were found dead with their tusks removed. The genotype of the seized tusk for five short tandem repeat (STR) loci is shown in Table 1. Allele names for each of the STR loci correspond to their size in base pairs (bp). Table 2 lists the genotypes of the same five STR loci for 10 recently slaughtered elephants. C. Did the seized tusk come from one of the recently slaughtered elephants? If so, which one, or if no, why not? The seized tusks came from elephant #8. This is because the seized elephants matches Perfectly with the decreased PCR. Since everyone has DNA that is unique, it was an obvious match- a. b. For each of the 5 STR loci, draw the expected pattern of bands on the gel below. Show the alleles for the seized tusk and the elephant you identified as a potential match. Also, include a DNA ladder that has DNA fragments that are 75, 125, 150, and 175 base pairs (bp) in size. Base Pairs 200 180 160 140 120 DNA Ladder CHR Seized Tusk Matching Elephant vollidsdongs 201 Seized Tusk FH19 FH60 Allele 1: Allele 2: Elephant 1 T able 1: Genotype for five STR loci recovered from a seized elephant tusk. Elephant 2 Elephant 3 Elephant 4 Elephant 5 Elephant 6 Elephant 7 FH67 FH71 193 147 97 62 193 147 103 62 Elephant 8 Elephant 9 Elephant 10 FH19 FH67 FH71 189 62 193 64 185 62 193 62 183 62 185 147 64 193 147 62 193 64 187 62 187 64 187 64 193 64 193 60 193 62 193 62 193 147 103 62 193 147 97 62 193 147 97 62 189 147 95 62 193 147 105 62 FH60 147 97 147 97 147 95 147 147 95 97 91 147 105 145 95 147 95 145 91 147 97 149 97 151 97 147 97 FH129 152 160 103 FH129 152 154 152 152 152 162 152 152 152 160 152 160 156 160 152 160 160 160 152 156 T Table 2: Genotypes using five STR loci for 10 slaughtered elephants. How does the pattern of bands in the gel support your conclusion about which elephant was the source of the seized tusks?
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