) Calculate the required gram of Tris-Hcl to prepare 1M, 100 ml Tris-HCl (Tris Stock) with mol and Molarity formula.
) Calculate the required gram of Tris-Hcl to prepare 1M, 100 ml Tris-HCl (Tris Stock) with mol and Molarity formula.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
1) Calculate the required gram of Tris-Hcl to prepare 1M, 100 ml Tris-HCl (Tris Stock) with mol and Molarity formula.
2)Calculate the required gram of EDTA to prepare O.5M, 100 ml EDTA (EDTA stock) mol and Molarity formula.
3.) Calculate the required volume of Tris Stock to prepare buffer A which contains 25 mM Tris-Hcl with final volume is 100 ml by using M1V1 =M2V2 formula
4) Calculate the required volume of EDTA stock to prepare buffer A which contains 10 mM EDTA with final volume is 100 ml by using M1V1 =M2V2 formula
5)Do the same for Buffer B calculations
Transcribed Image Text:Please make the following calculations for each buffer. Your calculations must be clear.
Do not forget to show the intermediate steps.
Each buffer calculation includes 2 parts. In the first part you need to show the calculations of
the stock solutions. In second part, you will show how you can prepare (by calculations) the
Buffer by using the stock solutions.
Pay attention to the desired volumes for each buffer. It is because you must make most of your
calculations according to these volumes.
Molecular weights
Tris base: 121,14 g/mol
EDTA disodium salt: 292,25 g/mol
NaOH: 39,997 g/mol
BUFFERS;
Buffer A 25 mM Tris-HCl, 10 mM EDTA (100 ml)
▪ 1 M Tris-HCl pH 8.0 (100 ml).
▪ 0.5 M EDTA (100 ml)
Buffer B 400 mM NaOH, 2% SDS (100 mL)
■
800 mM NaOH (100 ml).
4% SDS (100 ml)
These are the stock solutions
These are the stock solutions
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