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Consider the following table of restriction enzyme recognition sites. The / indicates where the enzyme cuts. A plasmid has a single Sphl site. This plasmid is cut with Sphl, and combined with genomic DNA digested (in separate reactions) individually with each of the enzymes in the table. Which of these digests of gDNA will produce fragments that can be cloned (ligated) into your Sphl digested vector; i.e., which have compatible cohesive ends? Indicate YES or NO in the third column for the remaining four enzymes, where YES means will ligate, and NO means will not ligate. Restriction enzyme Sphl Nlalll Ncol Nspl Pfl2311 Recognition site 5'-GCATG/C-3' 3'-C/GTACG-5' 5'-CATG/-3' 3'-/GTAC-5' 5'-C/CATGG-3' 3'-GGTAC/C-5' 5'-ACATG/T-3' 3'-T/GTACA-5' 5'-C/GTACG-3' 3'-GCATG/C-5' Compatible with Sphl? YES NO YES NO YES

Consider the following table of restriction enzyme recognition sites. The / indicates where the enzyme cuts. A plasmid has a single Sphl site. This plasmid is cut with Sphl, and combined with genomic DNA digested (in separate reactions) individually with each of the enzymes in the table. Which of these digests of gDNA will produce fragments that can be cloned (ligated) into your Sphl digested vector; i.e., which have compatible cohesive ends? Indicate YES or NO in the third column for the remaining four enzymes, where YES means will ligate, and NO means will not ligate. Restriction enzyme Sphl Nlalll Ncol Nspl Pfl2311 Recognition site 5'-GCATG/C-3' 3'-C/GTACG-5' 5'-CATG/-3' 3'-/GTAC-5' 5'-C/CATGG-3' 3'-GGTAC/C-5' 5'-ACATG/T-3' 3'-T/GTACA-5' 5'-C/GTACG-3' 3'-GCATG/C-5' Compatible with Sphl? YES NO YES NO YES

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Consider the following table of restriction enzyme recognition sites. The / indicates where the enzyme cuts. A plasmid has a single Sphl site. This plasmid is cut with Sphl, and combined with genomic DNA digested (in separate reactions) individually with each of the enzymes in the table. Which of these digests of gDNA will produce fragments that can be cloned (ligated) into your Sphl digested vector; i.e., which have compatible cohesive ends? Indicate YES or NO in the third column for the remaining four enzymes, where YES means will ligate, and NO means will not ligate. Restriction enzyme Sphl Nlalll Ncol Nspl Pfl2311 Recognition site 5'-GCATG/C-3' 3'-C/GTACG-5' 5'-CATG/-3' 3'-/GTAC-5' 5'-C/CATGG-3' 3'-GGTAC/C-5' 5'-ACATG/T-3' 3'-T/GTACA-5' 5'-C/GTACG-3' 3'-GCATG/C-5' Compatible with Sphl? YES NO YES NO YES
Transcribed Image Text:Consider the following table of restriction enzyme recognition sites. The / indicates where the enzyme cuts. A plasmid has a single Sphl site. This plasmid is cut with Sphl, and combined with genomic DNA digested (in separate reactions) individually with each of the enzymes in the table. Which of these digests of gDNA will produce fragments that can be cloned (ligated) into your Sphl digested vector; i.e., which have compatible cohesive ends? Indicate YES or NO in the third column for the remaining four enzymes, where YES means will ligate, and NO means will not ligate. Restriction enzyme Sphl Nlalll Ncol Nspl Pfl2311 Recognition site 5'-GCATG/C-3' 3'-C/GTACG-5' 5'-CATG/-3' 3'-/GTAC-5' 5'-C/CATGG-3' 3'-GGTAC/C-5' 5'-ACATG/T-3' 3'-T/GTACA-5' 5'-C/GTACG-3' 3'-GCATG/C-5' Compatible with Sphl? YES NO YES NO YES
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