Detail explanation on next generation sequencing

Q: Explain why exome sequencing can be almost as valuable as genome sequencing.

A: Whole genome sequencing refers to technique which used to analyze complete genome of an organism at…

Q: Why is genome database and annotation relevant to Genome sequencing?   Are there any examples?

A: Genome: the set of genes. Genome database: it is the collection of all information on the human…

Q: Which is a limitation of using massively parallel sequencing ?

A: It is a term used to describe the method of high-throughput DNA sequencing to determine the entire…

Q: Describe at least five (05) different types of commercially available vectors for cloning with size…

A: Cloning vectors are small DNA parts that are stably maintained in an organism. The foreign fragment…

Q: What is exome sequencing used for?

A: DNA sequencing:   It is a method to find a sequence of nucleotides by using the DNA sequencing…

Q: Differentiate between Sanger sequencing and next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: Using illustrations describe the structure of a typical cloning vector and discuss the functions of…

A: "Biotechnology" is the use of our knowledge of biological processes to the development of beneficial…

Q: list and explain two strategic between the shotgun sequences/ assemble strategy for whole genome…

A: Genomic Sequencing is a process where whole genome of an organism is sequenced. With the help of…

Q: Discuss the bioinformatics approaches that can be used to identifya protein-encoding gene.

A: The process of recognizing the protein-encoding gene is crucial and has numerous applications. Such…

Q: Explain the development of next generation sequencing (NGS)technology ?

A: The words next-generation sequencing (NGS), massively parallel sequencing (MPS), and deep…

Q: What are the vectors used in rDNA technology? briefly explain each

A: rDNA technology or Recombinant DNA technology is a widely used technology with the advancement of…

Q: Discuss about Genome 10K Project ?

A: The advent of low cost, high throughput sequencing has ushered in a new age of genome science and…

Q: Compare tblastx and tblastn software packages of genome sequence analysis.

A: BLAST stands for Basic Local Alignment Search Tool •What makes BLAST so popular??- Good balance of…

Q: Outline the general strategy used in metagenomics.

A: Metagenomics is otherwise known as environmental genomics, ecogenomics due to obvious reasons as it…

Q: Explain how DNA microarrays are used in the study ofgenomics.

A: DNA (deoxyribonucleic acid) is double helix, coil structure that is responsible for transfer of…

Q: How the Sequencing Technologies Have Progressed Rapidly ? Explain about this ?

A: Early efforts at sequencing genes ere troublesome, when Gilbert and Maxam reported the sequence of…

Q: High throughput whole genome sequencing, RNA-seq and ChIP-seq have all been made possible by the…

A: Genetic technologies have achieved great advancements in the recent years. One amongst many of those…

Q: Name any two cloning vectors. Describe the features required to facilitate cloning into a vector.

A: Cloning is a process that allows scientists to create perfect genetic duplicates of living…

Q: Write an accurate and precise differential report on the next generation sequencing technique

A: The biological sciences have been transformed by next-generation sequencing (NGS). NGS helps…

Q: Give three important characteristics of cloning vectors.

A: Cloning is an important process in which bacterial cells containing recombinant DNA can be readily…

Q: in your word, identify one difference one similarity between 16 S sequencing and metagenomic shotgun…

A: With the advancements in science, different techniques have emerged to sequence the genomes of…

Q: fill ups • Genome browsers show the arrangement and structure of_____ within RefSeq genomes.

A: A genome browser is a tool in bioinformatics which is used to display biological information in…

Q: Define about Sanger sequencing ?

A: Introduction:- The process of determining the sequence of nucleotide bases (As, Ts, Cs, and Gs) in a…

Q: Explain why a selectable marker is frequently included in a cloning vector.

A: A cloning vector is a short bit of DNA that may be kept in an organism for a long time and into…

Q: What are the advantages of Next Generation Sequencing?

A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…

Q: Give a step by step method and diagram of how reversible terminator sequencing works.

A: The reversible terminator sequencing or Illumina dye sequencing is a technique used to determine the…

Q: Discuss the principles , uses, advantages and disadvantages of illumina sequencing method

A: DNA copies obtained from PCR is subjected for gene sequencing through different gene sequencing…

Q: What is the difference between whole exome and whole genome sequencing?

A:

Q: What are the differences between somatic and germline gene editing? Is there any scientific example…

A: Gene therapy is a widely used technique to treat genetic disorders. There are two types of gene…

Q: Explain the impact of chromosome encoding in Genetic algorithms

A: Alleles are the smallest data units in a chromosome. In hereditary calculation, an encoding capacity…

Q: Refer to the figure. What method would you use if you wanted to determine the sequence of the cDNA…

A: cDNA is complementary DNA. This DNA is artificially synthesized from the mRNA template. In genetic…

Q: PLease explain What characteristics of plasmids makes them good cloning vectors? Discuss

A: Plasmids are small extrachromosomal DNA molecules that can reproduce independently of their…

Q: Describe the whole-genome shotgun strategy

A: BASIC INFORMATION SHOTGUN SEQUENCING In this random DNA strands are sequenced. It is named so…

Q: Review DNA sequencing and cloning tools. Match term and its description. main technologies for…

A: DNA sequencing is the process of identifying and writing the code of the whole genome in exact…

Q: Explain how to determine whether a given clone contains all or only part of the gene ?

A: Gene cloning is described as the process that includes a relocation of gene of interest and which is…

Q: Provide five advantages of Next Generation Sequencing? and explain each of these advantages.

A: Here I will discuss about the advantages of next generation sequencing.

Q: Traditional Sanger sequencing has largely been replaced in recent years by next-generation and…

A: BASIC INFORMATION  Gene Sequencing  It is a process through which the arrangement of of the…

Q: What is the program Finch TV used for? Viewing next generation sequencing data O Opening sequencing…

A: There are so many computer application programs used in bioinformatics to read and interpret the…

Q: How accurate is whole exome sequencing?

A: The goal of exome sequencing, also known as whole genome sequencing is to identify genetic variants,…

Q: Explain why a cloning vector usually includes a selectable marker.

A: A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into…

Q: List and explain the steps of DNA cloning using pUC8.

A: Introduction Molecular cloning is a set of molecular biology experimental methods for assembling…

Q: Explain why exome sequencing can be almost as valuable as genome sequencing. (Explain in your own…

A: Exome sequencing is one of the molecular tool to analyze the genes encoding for protein in entire…

Q: Describe the important features of cloning vectors. Explain the purpose of selectable markers in…

A: Cloning vector refers to small piece of deoxy ribonucleic acid (DNA), which are used to propogate…

Q: List the steps to make a genomic library. What steps would change if you wanted to make a cDNA…

A: You have asked two questions. The first one has been answered. To get the answer for the second…

Q: Discuss what is meant by the term genome

A: Organisms are classified as monoploid, diploid, triploid, tetraploid, etc based on the number of…

Genetic Evolution

Sexual reproduction produces distinct population variations. Heredity determines the traits and qualities to be passed down to the progeny. Over time, this variation within-species is also affected by the process of human evolution. The study of how genetic variation leads to evolutionary changes is known as genetic evolution. If a trait helps an organism to survive and reproduce, it is more likely to be passed down the generations. It is rare that such traits result in a completely distinct set of traits in descendants than the ancestral population; this can result in the emergence of a new species.