Examine the given sets of table below. Which disinfectant is the MOST recommended? Support and Justify your answ Table 2. Phenol Coefficient of Test Disinfectant Name of disinfectant Growth after 5 minutes Dettol Phenol Name of disinfectant Z-germicSide Phenol Dilution 1:100 1:125 1:150 1:175 1:200 1:05 1:100 1:105 1:110 1:115 Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 Growth after 5 mins Growth after 10 minutes Growth after 10 minutes * + ▾ Growth after 15 minutes * + + Growth after 15 mins
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- Examine the given sets of table below. Which disinfectant is the MOST recommended? Support and Justify your answer. Table 2. Phenol Coefficient of Test Disinfectant Name of disinfectant Growth after 5 minutes Dettol Phenol Name of disinfectant Z-germicSide Phenol Name of disinfectant Jik Phenol KEY:- = No growth, + = Growth Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 + + + + + + + + Growth after 5 mins + + + + + + + Growth after 5 mins + + + + + + Growth after 10 minutes + + + + + Growth after 10 minutes + + + + + Growth after 10 mins + - + + + + + + Growth after 15 minutes + + + + + + Growth after 15 mins - + + + + + Growth after 15 mins + + + + - + + + +What is the minimal concentration (MIC) for disinfectant number 1? Just write the corresponding number including the decimal as written. ug/mL. Disinfectant 1 2 3 4 concentration 0.1 ug/mL +++ ++++ ++ +++ 0.2 ug/mL ++ ++ + ++ 0.5 ug/mL + + 1.0 ug/mL 5.0 ug/mL subculture 1 2 3 4 0.1 ug/mL ++++ ++++ ++++ ++++ 0.2 ug/mL ++++ ++++ ++++ ++++ 0.5 ug/mL ++++ ++++ ++++ 1.0 ug/mL ++++ 5.0 ug/mL Submit responseGiven the following media recipe, pick the terms that apply (pick as many as are applicable). 10.0 g Meat extract 10.0 g Peptone 5.0 g Sodium chloride 15.0 g 1.0 L Agar Water Once media is cool, add 6% sterile sheep's blood Defined Complex Minimal Selective Differential
- Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:G When is it appropriate for gloves X + rface/defaultui/player/modern.html?configuration=&preventRightClick=False&cc=en-US&ieCompatibilityMod... 2 Exit rientation to Infection Prevention Which of the following are proper disinfection steps? Check all the apply. O Remove organic matter O Disinfect only O Follow suggested contact time O Mix cleaning products to make the disinfectant more powerful O Disinfect O Disregard the product labels SUBMIT ENG INChoose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible
- 5 1 2 6 Testing Anti-Microbial Agents: Disk-Diffusion. Lab Report Fill out the zones of inhibition table with your data: Agent Tested Cleansing Spray 3 4 Foaming Handwash Hand Sanitizer Lysol spray Detergent Desinfecting zone of inhibition: (mm) E. coli 10mm 15 mm 20mm 0mm 9mm P. aeruginosa 8 mm 35mm 17mm 15mm 0 mm 1mm Hydrogen Peroxide Here, write a few observations about your experimental results. 25mm S. aureus 25mm 20mm 25mm 12mm 8mm 42mm What factors (at least 2) other than the identity of the agent being tested can influence the size of the zone of inhibition? NOT Were any colonies observed within the zone of inhibition? Even if you didn't have any, explain why you might see colonies in the zone of inhibition. Be specific about how that might happen? What follow up questions or conclusions do you have related to your data? (at least 2)Below is a diagram of the serial dilution of a culture with 1 x 10º cells. Fill out the missing information. 250 Culture Diluent 1 x 10° cells/mL 9 mL 9 mL 9.9 mL 9.9 mL 99.9 mL Volume to add to diluent Final Dilution level Theoretical count after plating 100 uLMatch the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungi
- a physician prescribed an antibiotic to be mixed in white petrolatum to produce a 25% antibiotic preparation. The pyscian later changed the protocol to be only 12.5%. How much white petrolatum must be mixed with each 6-oz ointment container of 25% prepartation to make the new 12.5% preparation? Calculate answer in grams. Answer should be 180gA physician orders 1800 mL of lactated Ringer’s solutions to be administered over a 14 hour period. The IV is calibrated to deliver 12gtt/mL. How many drops per minute should the patient receiveSuspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (1210C) for 15 minutes. Cool to 45-500C. If desired the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri dishes. 1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses.