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- What is this result telling me? The ANCOVA confirmed the hypothesis that the antimicrobial dose response was paralleled between two bacterial species (S. typhimurium and E. faecium), and the interaction between antimicrobial concentrations and bacterial species was not significant (P > 0.05); only the antimicrobial concentrations of H2O2-PAA mixer played a significant (P < 0.05) impact on the inactivation of S. typhimurium and the surrogate bacteria E. faecium on squash.essential oils have been studied as potential antimicrobial agents. These naturally occuring compounds are linked to having the following type of activity in clinical trials: virucidal, septicidal, bactricidal, fungicidalMicrobial secondary metabolites are compounds produced by strains of certain microbial(antimicrobial agent).such as penicillin and cephalosporin. Describe the industrial fermentation process of each of these antibiotics. 1.media preparation (carbon source, nitrogen source, salts, oxygen requirements),Temperature and pH maintained.
- . A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium? a. Name four categories that blood agar fits. Alpha hemolysis Beta hemolysis Gamma Alpha prime Name four differential reactions that TSIA shows. Observe figure 3.15. Suggest what causes the difference in growth pattern between nonmotile and motile bacteria. Explain what a medium that is both selective and differential does, using figure 3.18. a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? MSA Mannitol salt agar One that lives in the human stomach? Why are intestinal bacteria able to grow…IN YOUR OWN WORDS. Differentiate the following. (Not more than 10 sentences) 1. Aseptic Teachnique vs. Sterile Technique 2. Microbistatic Agent vs. Microbicidal Agent 3. Sterilization vs. Disinfection 4.How is radiation, autoclave and ultrasonic waves used in the hospitals or clinics as a means to inhibit the growthbof microbes? 5. How is filtration applied nowadays in relation to Covid-19?3- Visible Growth in Tubes Growth in Petri Dishes 0 µg/ml 2 µg/ml 0 1 3 4 ÖÖÖÖÖÖÖÖ 2 5 6 7 number 1, and After addition of The figure given above summarizes an experiment carried out to test the efficacy of an antimicrobial substance. In the tube number 0, no antimicrobial substance was added into bacterial culture. 2 µg/ml of antimicrobial substance was added into tube the amount of antimicrobial was doubled for each consecutive tube. antimicrobial into tubes, tubes were incubated for overnight and after incubation tubes were observed for growth. The growth status of bacteria in each tube was shown as (+) and (-). In addition to observation of visible growth, 1 ml of sample from each tube was collected and plated into petri dishes and incubated for additional overnight. Similarly, the growth status in petri dishes was as (+) and (-). According to given information; a. Determine the minimum inhibitory concentration (MIC). b. Determine the minimum bactericidal concentration (MBC). c.…
- and the minimum exposure time 1. Determine the minimum inhibitory concentration given the following data: Concentration of ethanol Number of colonies of E. coli on Nutrient Agar plates Exposure time (in minutes) 10 5 15 Distilled water +++ +++ +++ 40% +++ ++ + 70% 95% Minimum inhibitory concentration: Minimum exposure time:Discuss why microorganism presents in the nature environment has a longer lag phase and shorter exponential phase as compared to laboratory cultured microorganism.Description 1. The fractions obtained from differential centrifugation are enriched but not pure. Explain how a greater degree of purification can be achieved using Density-gradient centrifugation and velocity centrifugation. 2. Explain equilibrium centrifugation.
- discuss the unique capacity of the Deinococci to tolerate desiccation and high dosses radiationBriefly explain this statement "Antineoplastic natural products- Taxol".please explain at your own words.In a fed-batch culture operating with intermittent addition of lactose solution, values ofthe following parameters are given at time t = 2, when the system is at a quasi-steady state. 1.Determine the initial volume of the culture 2. Determine the concentration of growth-limiting substrate and the total amount ofbiomass in the vessel at a quasi-steady state. 3. At which scenario of bioprocessing, a fed-batch system is recommended to be applied?