Visible Growth in Tubes Growth in Petri Dishes 0 μg/ml 2μg/ml 0 + 1 2 + + + + 3 + 5 6 0000 a. Determine the minimum inhibitory concentration (MIC). b. Determine the minimum bactericidal concentration (MBC). c. Propose a method to reduce MIC and MBC. 4 7 number 1, and After addition of The figure given above summarizes an experiment carried out to test the efficacy of an antimicrobial substance. In the tube number 0, no antimicrobial substance was added into bacterial culture. 2 µg/ml of antimicrobial substance was added into tube the amount of antimicrobial was doubled for each consecutive tube. antimicrobial into tubes, tubes were incubated for overnight and after incubation tubes were observed for growth. The growth status of bacteria in each tube was shown as (+) and (-). In addition to observation of visible growth, 1 ml of sample from each tube was collected and plated into petri dishes and incubated for additional overnight. Similarly, the growth status in petri dishes was as (+) and (-). According to given information;
Visible Growth in Tubes Growth in Petri Dishes 0 μg/ml 2μg/ml 0 + 1 2 + + + + 3 + 5 6 0000 a. Determine the minimum inhibitory concentration (MIC). b. Determine the minimum bactericidal concentration (MBC). c. Propose a method to reduce MIC and MBC. 4 7 number 1, and After addition of The figure given above summarizes an experiment carried out to test the efficacy of an antimicrobial substance. In the tube number 0, no antimicrobial substance was added into bacterial culture. 2 µg/ml of antimicrobial substance was added into tube the amount of antimicrobial was doubled for each consecutive tube. antimicrobial into tubes, tubes were incubated for overnight and after incubation tubes were observed for growth. The growth status of bacteria in each tube was shown as (+) and (-). In addition to observation of visible growth, 1 ml of sample from each tube was collected and plated into petri dishes and incubated for additional overnight. Similarly, the growth status in petri dishes was as (+) and (-). According to given information;
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 2.1P
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Visible Growth in Tubes
Growth in Petri Dishes
0 µg/ml 2 µg/ml
0 1
3
4
ÖÖÖÖÖÖÖÖ
2
5 6 7
number 1, and
After addition of
The figure given above summarizes an experiment carried out to test the efficacy of
an antimicrobial substance. In the tube number 0, no antimicrobial substance was added
into bacterial culture. 2 µg/ml of antimicrobial substance was added into tube
the amount of antimicrobial was doubled for each consecutive tube.
antimicrobial into tubes, tubes were incubated for overnight and after incubation tubes were
observed for growth. The growth status of bacteria in each tube was shown as (+) and (-). In
addition to observation of visible growth, 1 ml of sample from each tube was collected and
plated into petri dishes and incubated for additional overnight. Similarly, the growth status in
petri dishes was as (+) and (-).
According to given information;
a. Determine the minimum inhibitory concentration (MIC).
b. Determine the minimum bactericidal concentration (MBC).
c. Propose a method to reduce MIC and MBC.
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