How does the predicted molecular weight of AlaE (16949.07) compare with the molecular weight of the unmodified protein in the Western Blot attached? Are they consistent?

Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter3: Biosignals And Noise
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How does the predicted molecular weight of AlaE (16949.07) compare with the molecular weight of the unmodified protein in the Western Blot attached? Are they consistent? 

kDa
55 Cysless
35
20
10
F16C
E39C
L860
G125C
+++
+P
←U
M18C
V51C
+ +
Y96C
+
F139C
+
+++
M36C
K72C
-+MTSES
+ NEM
+++mPEG5K
+P
|D106C
+U
R143C
+P
-+MTSES
+- NEM
+++mPEG5K
◆U
+P
+U
+ MTSES
+ - NEM
+++mPEG5K
+P
TU
- + MTSES
- NEM
+++mPEG5K
+++
Fig. 1 SCAM analysis of AlaE. Western blot analysis of whole cell
lysates from cells harbouring plasmids expressing cysteine-free
('Cysless') or single cysteine variants of alaE with and without treatment
with NEM, MTSES and mPEG5K. PEGylated (P) and unmodified protein
are indicated with arrows.
Transcribed Image Text:kDa 55 Cysless 35 20 10 F16C E39C L860 G125C +++ +P ←U M18C V51C + + Y96C + F139C + +++ M36C K72C -+MTSES + NEM +++mPEG5K +P |D106C +U R143C +P -+MTSES +- NEM +++mPEG5K ◆U +P +U + MTSES + - NEM +++mPEG5K +P TU - + MTSES - NEM +++mPEG5K +++ Fig. 1 SCAM analysis of AlaE. Western blot analysis of whole cell lysates from cells harbouring plasmids expressing cysteine-free ('Cysless') or single cysteine variants of alaE with and without treatment with NEM, MTSES and mPEG5K. PEGylated (P) and unmodified protein are indicated with arrows.
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