Luther found a vial containing an unknown lipid sample. He separated the different lipids using gel filtration chromatography and determined the identities of the different lipid eluents using normal phase TLC. Help Luther complete the table below. Report the distance travelled of eluents and solvent in two decimal places and the R, in four decimal places. For the identities of the lipids, refer to the provided pool of chemical structures and write only the letter code.
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- You want to prepare a 15 mL gel containing 0.8% (weight /volume) agarose. How much agarosedo you need to weigh to prepare the solution? Show all your calculations.Hint: Write down the units for 0.8% knowing that agarose is a powder that is mixed with aliquidWill the given lipid test positive in the Ninhydrin test?Identify components of an unknown sample I An unknown liquid sample is tested for chemical composition. You receive the following results: iodine test=yellow; biuret test = blue; Benedict's test = blue; and paper lipid test = clear. Which of the following are present in this sample? (Select all that apply) Check All That Apply starch protein sugar lipids None of these are present.
- A mixture of lipids containing phosphatidic acid, cholesterol, testosterone, phosphatidylserine, andphosphatidylethanolamine was applied to a hydrophobic interaction chromatography column. Thecolumn was washed with a high salt buffer, and the lipids were then eluted with decreasing saltconcentrations. In what order would the lipids be eluted from the column? Explain your answer.GRA H-C-OH C=0 HO-C-H H-C-OH H-C-OH H-C-OH CH₂OH H HH HO Select the correct molecule for each of the following questions. You may use each OH H H OH -CH₂ 0-P-0- 0 40 40 EEEEEEEEEE CCCCCCCCCCE AXCCCCCES voor m dA mixture of lipids is applied to a silica gel column (a column chromatography), and the column is then washed with increasingly non-polar solvents. The mixture consists of phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, cholesteryl palmitate (a sterol ester), sphingomyelin, palmitate, n-tetradecanol, triacyglycerol, and cholesterol. In what order will the lipids elute from the column? Explain the reasons for your answer(s).
- A mixture of lipids is applied to a silica gel column, and the column is then washed with increasingly polar solvents. The mixture consists of phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, cholesteryl palmitate (a sterol ester), sphingomyelin, palmitate, n-tetradecanol, triacylglycerol, and cholesterol. In what order will the lipids elute from the column? Explain your reasoningLipids from an organic sample are extracted separately by column chromatography using the ff eluants: 1st eluant - chloroform: methanol: water (6:3:1) 2nd eluant - methanol: chloroform (9:1) 3rd eluant – petroleum ether: ethyl ether (1:1) Test results for each eluate are tabulated below. Identify the possible lipid present in each eluate. Test Reagents 1. KHSO4 2. HNO3,(NH4)3MoO4 3. CHCl3, acetic anhydride, conc. H2SO4 4. -naphthol, conc. H2SO4 5. Hydroxylamine HCl, FeCl3 6. ninhydrin reagent POSSIBLE LIPIDS: Triacylglycerol, cholesterol, sphingomyelin, lecithinA mixture of lipids containing phosphatidic acid, cholesterol, testosterone, and phosphatidylcholine was applied to a hydrophobic interaction chromatography column. The column was then washed with increasingly nonpolar solvents. IWhat order would the lipids be eluted from the column?
- Refer to the following titration curve below: 13 12 11 10 7 6 5 4 4 6 8 10 12 14 16 18 20 22 24 26 28 30 Volume of Titrant / mL - Unknown Acid 0.10 mol/L - titrant = NaOH 0.1 mol/L What is the isoelectric point of histidine, according to the titration curve? Input your answer until the first decimal point. 2. 9,The following were obtained when testing for lipids. Discuss and conclude from the results obtained.Use the provided theoretical titration curve of histidine in answering the questions asked. pH 14 12 10 8 6 4 2 O 0 2 4 1. In the titration curve above, what is the isoelectric point of histidine? Make sure to place your answer up until the first decimal point. 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 Volume of Titrant / mL Histidine 0.10 mol/L - titrant = NaOH 0.1 mol/L 2. Which form of histidine is the most abundant at pH 10.0? (and why) a. Hist b. His c. His2+ d. His 3. True or False: All amino acids have zwitterions (explain) 4. Which of the following forms of Histidine is/are amphoteric? (More than one answer may apply) (explain) a. Hist b. His c. His2+ d. His