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I. How much of a 3X stock of SDS Loading Buffer should you add to 20 uL of sample to make 30 uL of 1X?
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- What would be the working stock concentration (in molarity) if you diluted 250 uL of a stock concentration of 10 uM in a total volume of 1 mL?BSA Concentration (ug/mL) Abs 2000 2.25 1500 1.715 1000 0.915 750 1.156 500 0.714 250 0.082 125 0.086 25 0.082 Create a BSA standard curve Abs562=f[BSA] using a Scatter Plot representation in Excel.If 400 mL of a 20% w/v solution were diluted to 2 L, what would be the finalpercentage strength?
- You need to prepare 1L of a 5×TE stock solution (1×TE buffer:10mM Tris,1mM EDTA).How much of Tris you need? How many ml of the 0.5 EDTA stock solution do you need?Calculate the volume of TAE buffer that you will need to prepare a 100ml solution of 1X strength if you only have a 50X concentrated solution available.how many grams of sr(no2)2 are needed to make 200 ml of 0.300M solution of sr(no2)2
- A 510 mL IV bag contains a 25% dextrose solution. How much of the original solution must be replaced with a 65% dextrose solution to increase the original concentration to 35%?If 4 mL of 1 M NaOH is added to 100 mL buffer, would it still be a usable buffer according to the conventions? Explain why or why not.If a pharmacist needs to prepare a bacterial suspension with a concentration of 2.4x10^2 cfu using 1 mL of 2.4 x 10^4 bacterial suspension, what size of volumetric flask will she/he be needing?
- 16. How many milliliters of a 0.9% (w/v) solution of sodium chloride can be pre- pared from 50 tablets, each containing 2.25 g of sodium chloride? 16. 12,500 mLcan you show how to calculate the amount of two components for the required volume of a buffer with the receipes? Prepare enough 10X stock solution § Prepare 1LProportions§ 0.25M tris§ 1.92M glycine§ pH 8.3§ 1% (w/v) SDSInstructions to prepare 100mL of 10X running buffer1. Weigh 3.02g of Tris base and 14.42g of glycine into ~90mL of ddH2O in a clean beaker and stir todissolve.2. Check the pH with a calibrated pH metre once dissolved.§ The pH of the buffer should be 8.3. pH adjustment is not required if between 8.1-8.5.3. Add 5mL 20% (w/v) SDS stock solution (a liquid) to make a 1% (w/v) solution.4. Top the volume to 100mL by adding 5mL ddH2O from a graduated cylinder.How much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)