Identify the possible issues if a sample in a spectrophotometer gives no reading. Select one or more: O The sample may be placed improperly in the cuvette holder. O The wrong wavelength may be set. O There may be an issue with the spectrophotometer. O There may be an issue with the composition of the sample.
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- Here are the materials and method for the basic spectrophotometer experiment Materials:1. Paper template2. Scissor3. Light source (i.e. torchlight, portable light)4. Paperboard (black colour)5. Unused compact disc (CD)6. Camera detector (i.e. webcam, laptop, mobile phone) Methods: 1. Make a foldable paper spectrophotometer using the paper template and followthe instruction as in https://publiclab.org/sites/default/files/8.5x11minispec3.8.pdf2. Then, mounted the paper spectrophotometer to the camera detector.3. Download any freely available spectral analyzer.4. Test and observe the wavelength with and without the presence of a light source.5. Finally, observe the wavelength with a different transparent colour sheets (you canchoose any colour). Troubleshooting/ problem(s) encountered for basic spectrophotometer experiment.What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective lens? O 45x O 100x 4.5x O 450x O 10xWhich of the following statements is/are TRUE? Kindly choose all that applies. Select the correct response(s): When FOV increases, the specimen magnification decreases. When FOV increases, the light intensity increases. When FOV increases, the specimen magnification increases. When FOV increases, the light intensity decreases.
- Which of the following is/are source/s of error in performing a spectrophotometric method? Answer all that apply. Sample is turbid when the absorbance is read. The absorbances were read at maximum absorption. Standard solutions are prepared accurately. The cuvettes have fingerprints.A michroscope has a 20x ocular and two objectives of 10x and 43x respectively: calculate the apparent magnification for low and high power lenses.e) What is the total magnification of a specimen when viewed with the following objective: 400 Scanning High Power 400 Low power Oil Immersion
- when you want to compute for the magnification of the specimen, what will you do with the eyepiece and the given objectives magnificationWhich of the following statements are true about the use of a blank in spectrophotometry? Select all that apply. Select 2 correct answer(s) A blank is needed because the spectrophotometer also measures the absorbance of the cuvette and water. A blank must be measured prior to measuring your sample. None of the answer choices listed are correct. A blank must be measured after measuring your sample. A blank is only needed when the concentration of your sample is known.What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?
- Describe the process to get a specimen into view using the 40X objective starting from the point after you have plugged in your scope and cleaned the objectives and slide.What information can be obtained from the slides prepared by the wet mount technique and the hanging mount technique? Give at least three (3) information. What are the limitations of each method (Provide two limitations)How does the numerical aperture of an objective lens affect resolution? Question options: Numerical aperture is the optical plane visible for each objective lens Numerical aperture decreases the amount of excess light on a specimen increasing the resolution and clarity Numerical aperture is the loss of light due to changes in density between the specimen and the objective lens Numerical aperture is a measure of the amount of light an objective lens can accept producing higher resolution and allowing smaller structures to be visualized with greater clarity