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- 1 Restriction enzymes cut DNA molecules atspecific locations to produce restriction fragments witheither blunt or _______ endsFIGURE 2 shows the only suitable DNA restriction site in a plasmid DNA vector that can be cleaved. 5'.GTAATCCGGATCCGAAATCCCCCGG... 3' 3.CATTAGGCCTAGGCTTTAGGGGGCCC... 5 FIGURE 2 Identify the most suitable enzymeto be used as a restriction enzyme forthis purpose. O Ligase O Smal O EcoRI О ЕсoRVAny change in WT DNA sequence without any phenotypic appearence Mutant Mutation Genotype Phenotype
- Which of the restriction enzymes listed in the table below produces blunt-end fragments? Enzyme Cleavage site Alul AG|CT Hindll AJAGCTT BamHI G|GATCC EcoRI GJAATTC BgllI A|GATCA Alul O BamHI O Bell O EcoRI O HindlllExplain in detail the AMES test which is used to test for the mutagenic potential of different compounds. Include an explanation of the AMES tester strains and how they work, why the liver is essential to this test and specifically why the control plate is necessary.Most prokaryotic organisms produce one or more enzymes to defend themselves against the infection by foreign DNA. These enzymes are known as O DNA Ligase restriction enzymes O helicase O polymerase
- Explanation of why mutations vary in likelihood of causing disease depending on location relative to a gene Explanation of what is a genetic test Explanation of the PCR method ALL RELATES TO Phenylketonuria PLEASEEEEEE ANSWER ALLLL PLEASEEEEd. Biuret test O e. NaOH Clear my choice In the method to isolate the DNA from peas, you used the centrifuge right after the addition of salts and alcohol; in which part of the tube will you find the stringy white DNA? of Select one: stion a. Suspended in the solution O b. At this step, no DNA will be visible O c. Top of the tube precipitate O d. Bottom of the tube precipitate Clear my choiceCystic fibrosis (CF) test to screen for mutations associated with the CFTR gene. Positive samples will undergo gene sequence analysis. What assay format is used in the screening test.
- Which enzyme was used to produce the molecule in the figure above? O ligase O DNA polymerase O RNA polymerase O a restriction enzyme (endonuclease) O topoisomeraseConsider the following results from the Ames test: Test plate - 20 colonies Does the test plate contain a chemical that is a mutagen? O Not enough information is present to answer the question. Yes O NoWhich one of the following statements is FALSE regarding the Ames test? O liver enzymes are added to the assay so that it will mutate the DNA of the Salmonella bacteria O Agar plates that do not include histidine are used to examine whether the test substance has caused mutations O A control is necessary to determine the background level of mutation The suspected mutagen (test substance) is omitted from the negative control sample O The test is done to determine whether a particular chemical can cause a reversion of the mutant strain to the wild type phenotype
