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Protein-coding sequences are the only highly conserved regions in our genome
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- If the coding region of a gene (the exons) contains 2,100 base pairs of DNA, would a missense mutation cause a protein to be shorter, longer, or the same length as the normal 700 amino acid proteins? What would be the effect of a nonsense mutation? A sense mutation?Given the following tRNA anticodon sequence, derive the mRNA and the DNA template strand. Also, write out the amino acid sequence of the protein encoded by this message. tRNA: UAC UCU CGA GGC mRNA: protein: How many hydrogen bonds would be present in the DNA segment?Since it was deciphered four decades ago, somehave claimed that the genetic code must be a frozen acci-dent, while others have argued that it was shaped by nat-ural selection. A striking feature of the genetic code is itsinherent resistance to the effects of mutation. For example,a change in the third position of a codon often specifies thesame amino acid or one with similar chemical properties.The natural code resists mutation more effectively (is lesssusceptible to error) than most other possible versions, asillustrated in Figure Q1–1. Only one in a million comput-er-generated “random” codes is more error-resistant thanthe natural genetic code. Does the extraordinary mutationresistance of the genetic code argue in favor of its origin asa frozen accident or as a result of natural selection? Explainyour reasoning.
- Given the following genomic sequence which contains 2 exons and CDNA sequence predidt the amino acid sequence of the exons which are part of the coding region of a protein. How many nucleotides make up the intron? Second letter UAU TV UACJ UAA Stop|UGA Stop A UAG Stop UG Trp UUU1 Phe UUCJ UGU1, UGCJ UCU) Cys UCC Ser UCA ULA UCG CUUT CUC CUA CUG CCU CAUT C4C His CAAT CAGJ OGU CCC Leu CA CGC FArg CGA Gln CGG Pro CCG AUU AUC lle AUA ACU AGU AAU AACJ AAA 1 AAGYS Asn Ser ACC Thr ACA AGC. AGA Arg AUG Mer ACG, AGG GCU GAUT GACJ GGU GGC GUU Asp GUC Val GUA Gly Ala GAA Glu GAGJ GGA GGG GUG GCG G. Genomic antisense sequence: 5'-TAACTGATTCTAGGCTACTCTTTCACCTAA-3" CDNA sequence: 5'-TAACTGATTTCTTTCACCTAA-3" I Use the editor to format your answer First letter 皇 Third letterThe following segment of DNA codes a protein. The uppercase letters represent Exons, the lowercase letters introns. Draw the pre- mRNA, the mature mRNA and translate the codons using the genetic code to form the protein. Identify the 5’UTR and 3UTR 5’- AGGAAATGAAATGCCAgaattgccggatgacGGTCAGCaatcgaGCACATTTGTGATTTACCGT-3’One remarkable feature of the genetic code is that amino acids with similar chemical properties often have similar codons. thus codons with U or C as the second nucleotide tend to specify hydrophobic amino acids. Can you suggest a possible explanation for this phenomenon in terms of the early evolution of the protein-synthesis machinery?
- Assume the first nucleotide in the sequence is at the +1 position. Transcribe the DNA sequence into mRNA, and then translate it into the polypeptide. Give the polypeptide sequence in the following form: Met-Thr-Trp-Tyr-Val etc. 3' TCACAATACAAAGGTGTACTGATCTCATCTCCATAA 5'Below is a sequence of DNA.5'-ttaccgataattctctctcccctcttccatgattctgattaaagaaggcgagaacgaaactatttgttaatacc-3' How many "reading frames" can be identified for this sequence? How many "open reading frames" can be identified for this sequence? What is the frame of the longest ORF? How many codons are in the longest ORF? What is the frame of the shortest ORF? How many AA are in the shortest ORF? Using the one letter code for Amino Acids, what is the predicted AA sequence of the shortestORF (from N to C-terminal end)? Using the one letter code for Amino Acids, what is the predicted AA sequence of the longestORF (from N to C-terminal end)?A crime has occurred, and you are asked to help solve it. A forensics detective brings you a sample from the crime scene of what they believe is the suspect's DNA and asks you for chemical analy Your study of this sample reveals the presence of adenine, thymine, ribose, and uracil, leading you to conclude that the sample is A pure DNA probably a mixture of DNA and RNA C pure RNA probably a mixture of rRNA and mRNA MAR 12 tv MacBook Air DII DD esc F6 F7 F8 F9 F10 F1 F1 F2 F3 F4 F5 &
- The following DNA sequence is derived from the middle of an exon of a eukaryotic gene. This sequence encodes a seven amino acid stretch of a protein. 5' ATGCGGAТАААТТААAАТТСС 3' 3' TACGCCTATTTAATTTTAAGG 5' If the third base pair from the left in the DNA sequence were deleted, what type of mutation would this be called? Second Letter G. UUU UUUC UUA UUG Phe UCU UCC UCA UCG UAU UAC UAA UAG Tyr UGU UGC Stop UGA Stop A Stop UGG Trp G Ser Leu CUU C CUC CUA CUG CCU Leu CCC CCA CCG CAU His CGU CGC Arg CGA CGG Pro САС CAA CAG 1st Gin 3rd letter AUU A AUC AUA AUG ACU le ACC ACA Met ACG AAU AAC AAA AAG AGU AGC AGA AGG Asn Ser U letter Thr Lys Arg GUU G GUC GUA GUG GCU GCC GCA GCG Asp GAU GAC GAA GAG GGU GGC GGA GGG Val Ala Gly Glu nonsense O frameshift O dominant missense UCACD CAGU CAG UCACHow many PAM sequences are present in the sequence below? 5'-GCACGGCGGAGCGGTTCTTGGCAGCGGCCGCACGATCTCGTTGCCGCCGG-3' O 2 O 3 O 4 O5 0 6Which of these single strand RNA sequences could form a hairpin secondary structure? 5' AAAAAAAAAAAAAAAAAAA 3' 5' ACACACACACACACACAC 3' 5' CCCGGGGUUUUCCCCGGG 3' 5' UUUUUUUUUCCCCCCCCC 3' 5’ UUUGGGUUUGGGUUUGGG 3’
