Scanning electron microscope can be used for () A. Observe images of different sections of cells B. Observe living cells C. Quantitative analysis of chemical composition in ce D. Observation of cell surface stereomorphology E. Observation of cellular activity
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Q: human cheek cells
A:
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- A biologists is studying the internal ultrastructure of cells of unknown source. The biologist can achieve the finest resolution by using a А. compound light microscope В. scanning electron microscope С. transmission electronic microscope D. phase-contrast light microscope Based on your answer in the previous item, what is the drawback of using this type of microscope? А. provides higher contrast cannot view dynamic processes of the cell B. provides for higher resolving power С. provides for higher magnification D. What support can give to substantiate your answer in the previous item?Many microscopy techniques use cell characteristics, such as differences in absorption, refraction, or even light polarization. Fluorescence microscopy provides an image from a single molecular species, simply by looking at the fluorescence emission. How does fluorescence microscopy compare to other techniques? A.Image the location, diffusion or even transport of intracellular molecules.B.Accurately calculate the cytosol refractive index of a cellC.Count the number of cellsD.Accurately measure kernel sizeStaining of cells is often performed to enhance images acquired by brightfield microscopy. Phasecontrast microscopy does not require cell staining. Why is this advantageous?
- B. TYPICAL PLANT LEAF CELL (Western Pond Weed): Pond weed is a great plant for studying plant cells, because the leaves are only two cells thick and it is easy to make mounts and view the cells under the microscope. Use the "close the book" method to make a wet slide of one pond weed leaf. The instructor will demonstrate the procedure. Place the slide on a compound scope and use the "stair- step" procedure to focus on a few cells of the leaf at high power. You should definitely be able to see cell walls and chloroplasts. Rarely, you may see a nucleus. In some of the cells, you may notice that the chloroplasts are moving. They seem to cycle around the center of the cell. This is called cyclosis, and is caused by the cytoplasm streaming around the vacuole carrying some organelles with it. Draw and label ONE cell on the provided drawing area of the next page and label using the labels below: CELL WALL, CHLOROPLASTS, VACUOLE, NUCLEUS (if visible) CPID 810186 10Which electron or light microscopy technique would you use for the following? (also explain) For visualizing the fine structure of cellular organelles and cell architecture in the cell interior using very thin cross-sections made in Epon. To obtain sequential images of a macromolecule in order to study its activity in real-time. To obtain an extremely clear three-dimensional image of the nucleus of a single cell. What is the magnification of the image in a microscope using a 20x ocular and a 100x objective?The best definition for resolution is: O The ability of an organelle to pick up stain The thickness of a specimen to be examined by microscopy The observed size divided by the actual size O The magnification of a microscope The distance between two distinguishable objects
- Explain fluorescence microscopy can allow us to visualize cells but cannot help identify them is true or false and why?The 3 components that make a microscope useful in the realm of cell biology are contrast, resolution, and magnification True O FalseMeasure the length of Cell X using the ruler in Microsoft Word. Assuming the actual length to be 3 um, calculate the magnification. Show your complete solution. Based on Figure 1, what internal organization can be distinguished in cell X? Why? Based in Figure 1, can you see a limiting membrane? Can you deduce its presence? Why or why not?
- Briefly describe how magnetic bead microrheometry is used to measure the mechanical properties of cells.b) The dingram ahows some other cells. Size of image E magnitica tion s mm/e 35 mm. asmm 100OX. i) Give three features of theses cells which show that they were part of a plant. ii) Make a drawing of cell X. Your drawing must be four times larger than cells X in the diagram. 2. Which of the following is not part of an animal cell? Nucleus Cell membrane Cytoplasm Cellulose cell wall 3. One difference between green plants and animals is that green plants have Chloroplasts Cytoplasm Nucleus Cell membraneProvide a description of the observation of human cheek cells under high power objective in microscope.