Suppose you do an experiment in which you add to the inhibited culture (i.e., phosphoglucose isomerase is inhibited) the glucose shown below that is labeled with ¹3℃ at C3 and 14C at C4 as the sole carbon source (i.e., in this experiment the labeled glucose is the only nutrient). НО. 14C HO HT 13C OH OH -OH (a) Is ¹4CO₂ produced under these conditions? If so, what percentage of the total CO₂ produced is ¹4CO₂? (b) Is ¹3CO₂ produced under these conditions? If so, what percentage of the total CO₂ produced is ¹3CO₂? (c) Assume the only source of ethanol is the labeled glucose. Draw the various labeled ethanol molecules that are produced under the conditions described above. Clearly show where ¹³C and ¹4C appear in each of the different ethanol molecules. (d) What is the expected yield of ATP/glucose under these conditions (i.e., anaerobic, phosphoglucose isomerase is inhibited, and all glucose is converted to ethanol as described above). Explain your reasoning.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter27: Metabolic Integration And Organ Specialization
Section: Chapter Questions
Problem 20P: Figure 27.3 illustrates the response of R (ATP-regenerating) and U (ATP-utilizing) enzymes to energy...
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Suppose you do an experiment in which you add to the inhibited culture (i.e.,
phosphoglucose isomerase is inhibited) the glucose shown below that is labeled with ¹³℃ at C3 and
14C at C4 as the sole carbon source (i.e., in this experiment the labeled glucose is the only nutrient).
HO.
14C
OH
НО
13C
OH
OH
(a) Is ¹4CO₂ produced under these conditions? If so, what percentage of the total CO₂
produced is ¹4CO₂?
(b) Is ¹3CO₂ produced under these conditions? If so, what percentage of the total CO₂
produced is ¹3CO₂?
(c) Assume the only source of ethanol is the labeled glucose. Draw the various labeled
ethanol molecules that are produced under the conditions described above. Clearly show
where ¹³C and ¹4C appear in each of the different ethanol molecules.
(d) What is the expected yield of ATP/glucose under these conditions (i.e., anaerobic,
phosphoglucose isomerase is inhibited, and all glucose is converted to ethanol as
described above). Explain your reasoning.
Transcribed Image Text:Suppose you do an experiment in which you add to the inhibited culture (i.e., phosphoglucose isomerase is inhibited) the glucose shown below that is labeled with ¹³℃ at C3 and 14C at C4 as the sole carbon source (i.e., in this experiment the labeled glucose is the only nutrient). HO. 14C OH НО 13C OH OH (a) Is ¹4CO₂ produced under these conditions? If so, what percentage of the total CO₂ produced is ¹4CO₂? (b) Is ¹3CO₂ produced under these conditions? If so, what percentage of the total CO₂ produced is ¹3CO₂? (c) Assume the only source of ethanol is the labeled glucose. Draw the various labeled ethanol molecules that are produced under the conditions described above. Clearly show where ¹³C and ¹4C appear in each of the different ethanol molecules. (d) What is the expected yield of ATP/glucose under these conditions (i.e., anaerobic, phosphoglucose isomerase is inhibited, and all glucose is converted to ethanol as described above). Explain your reasoning.
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