The data in the table are used to create a calibration curve for the determination of RNA from ts absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the Dest straight line fit to the calibration curve. Do not subtract the blank reading when creating he calibration curve. m= b= RNA Absorbance at 260 (µg) 0.00 10.36 20.72 31.08 41.44 nm 0.268 0.497 0.795 1.131 1.373 µg-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.699.
The data in the table are used to create a calibration curve for the determination of RNA from ts absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the Dest straight line fit to the calibration curve. Do not subtract the blank reading when creating he calibration curve. m= b= RNA Absorbance at 260 (µg) 0.00 10.36 20.72 31.08 41.44 nm 0.268 0.497 0.795 1.131 1.373 µg-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.699.
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter5: Biomedical Electronics: Digital
Section: Chapter Questions
Problem 4P
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