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Using the results of linear regression analysis (log(MW, kDa) vs Rr) from the protein standards, calculate the molecular weight (rounded to nearest kDa) of each protein band by completing the table below. Table D.1. List of different protein bands from SDS-PAGE and their corresponding Rf values and molecular weights (kDa). Molecular Weight, kDa Protein Bands LANE 1 LANE 2 AR B C D A B D Rf 2.0950 2.4344 2.6299 2.9396 2.1761 2.2904 2.4858 2.6813 Results of Linear Regression Analysis: Parameters Slope: Y-intercept: R²: Value -1.5403 5.5279 098110777

Using the results of linear regression analysis (log(MW, kDa) vs Rr) from the protein standards, calculate the molecular weight (rounded to nearest kDa) of each protein band by completing the table below. Table D.1. List of different protein bands from SDS-PAGE and their corresponding Rf values and molecular weights (kDa). Molecular Weight, kDa Protein Bands LANE 1 LANE 2 AR B C D A B D Rf 2.0950 2.4344 2.6299 2.9396 2.1761 2.2904 2.4858 2.6813 Results of Linear Regression Analysis: Parameters Slope: Y-intercept: R²: Value -1.5403 5.5279 098110777

Curren'S Math For Meds: Dosages & Sol
Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 8.1P
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Using the results of linear regression analysis (log(MW, kDa) vs Rr) from the protein standards, calculate the molecular weight (rounded to nearest kDa) of each protein band by completing the table below. Table D.1. List of different protein bands from SDS-PAGE and their corresponding Rf values and molecular weights (kDa). Protein Bands A B C D A B C D LANE 1 LANE 2 Rf 2.0950 2.4344 2.6299 2.9396 2.1761 2.2904 2.4858 2.6813 Molecular Weight, kDa Results of Linear Regression Analysis: Parameters Slope: Y-intercept: R²: Value -1.5403 5.5279 0.98110777
Transcribed Image Text:Using the results of linear regression analysis (log(MW, kDa) vs Rr) from the protein standards, calculate the molecular weight (rounded to nearest kDa) of each protein band by completing the table below. Table D.1. List of different protein bands from SDS-PAGE and their corresponding Rf values and molecular weights (kDa). Protein Bands A B C D A B C D LANE 1 LANE 2 Rf 2.0950 2.4344 2.6299 2.9396 2.1761 2.2904 2.4858 2.6813 Molecular Weight, kDa Results of Linear Regression Analysis: Parameters Slope: Y-intercept: R²: Value -1.5403 5.5279 0.98110777
2. During SDS PAGE, Tighnari loaded the purified protein extract from redcrest and padisarah, another sample that he is working with for his dissertation. Note that the isolated proteins from padisarah have molecular weights similar to that of the proteins isolated from redcrest. The results of the SDS-PAGE are shown in Figure D.2. SDS-PAGE w/o ß-mercaptoethanol LANE 1 B D LANE 2 PHILGAR SDS-PAGE w/ p-mercaptoethanol LANE 1 !!! LANE 2 Figure D.2. Electrophoretogram obtained after SDS-PAGE of the purified protein extract from redcrest and padisarah.
Transcribed Image Text:2. During SDS PAGE, Tighnari loaded the purified protein extract from redcrest and padisarah, another sample that he is working with for his dissertation. Note that the isolated proteins from padisarah have molecular weights similar to that of the proteins isolated from redcrest. The results of the SDS-PAGE are shown in Figure D.2. SDS-PAGE w/o ß-mercaptoethanol LANE 1 B D LANE 2 PHILGAR SDS-PAGE w/ p-mercaptoethanol LANE 1 !!! LANE 2 Figure D.2. Electrophoretogram obtained after SDS-PAGE of the purified protein extract from redcrest and padisarah.
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