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- Consider the reaction below. Where would the labelled carbon (represented by the asterisk * in the reactant) end up in the product? CH₂OPO3² NADP+ 44 * OH OH OH as CO2 C-1 C-2 C-3 C-4 C-5 NADPH CO₂ 1 CH₂OH 2 3 :0 CHOH 4 CHOH 5 CH₂OPO3²-Complete the following diagram, using arrows to show the flow of electrons, for this reaction catalyzed by GAP dehydrogenase. Draw how the enzyme pocket appears as the reaction is completed. Indicate the product (if any). NAD+ ÇHOPO он СysWhich of the following statements is true for the shown reaction? The reaction is shared by ketone body synthesis and cholesterol biosynthesis The chemical in box B is NAD+ The reaction predominantly occurs in the liver All of the above None of the above
- The following figure shows two absorption spectra. NAD* is the solid line. NADH is the dashed line. 16 NAD+ 12 NADH 240 280 320 360 Wavelength (nm) What is the wavelength of maximal absorbance of NADH in the 280-360 range? Molar absorbance x 103Consider this chemical equation: Catalase 2H,02 2H,0 + O2 Hydrogen Water Oxygen peroxide What is the enzyme in this reaction? O catalase O2 O 2H2O2 2H20In this diagram, which of the following is TRUE? H H+ (proton) e- Organic molecule that includes two hydrogen atoms (H) ©2019 Pearson Education, Inc. NAD+ coenzyme (electron carrier) Oxidation Reduction O None of the other four answers are true O The NAD+ becomes reduced Oxidized organic molecule Neither molecule is oxidized or reduced The organic molecule becomes reduced The NAD+ becomes oxidized H+ NADH + H+ (proton) (reduced electron carrier)
- HOT Metabolite Substrate Product ATP ADP Pi NAD+ NADH FAD FADH₂ OH Concentration (mm) 0.318 0.224 4.95 0.825 0.724 2.98 1.73 1.42 0.843 HO HO Use the metabolite concentrations in the table to calculate the AG of the reaction above. Report your answer in kJ/mol to 3 significant figures. OHBalance the following net reaction that occurs during oxidative phosphorylation. Use the smallest whole integers possible for the reaction stoichiometry. NADH+ FADH₂ + ADP+ 0₂+H+PO, ¹ NAD+FAD+ ATP+ +H₂OConvert the following reactions into "Metabolic Engineering" notation: C6H1206 + 2 H20 → 2 C2H60 + 2 HCO3 + 2 H* b. ATP + glucose > glucose-6-phosphate + ADP 3.
- Example: Oxidation of ethanol by NAD+ in the presence catalyzed by alcohol dehydrogenase Calculate the standard free energy change for the reaction below: Ethanol + NAD* 2 acetaldehydye + NADH + H*Draw the major product of the following reaction. CH3 HNO3 H2SO4 H2, Ni NaNO2, H3O+ KCNRefer to the figure shown here, and determine the value of E for the overall oxidation/reduction reaction (refer to the book/lecture slides if you need help with the overall reaction). 121/202 + 2H+ + 2e → H₂0 NAD + H* + 2e → NADH O-1.136 volts O 0.496 volts O+1.136 volts voltsm -0.496 volts EU (volts) +0.816 - 0.320
