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When the aspirin is tested to see if it breaks down more efficiently in HCI or NaOH; a) why is a control test done using salicylic acid? b) what is the purpose of the FeCl3 in this
aspirin test?
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- a) What are the methods used for cholesterol determination? Explain the basis of the method you used in your experiment by writing.b) In the experiment conducted for the determination of cholesterol, the function of the calibration curve obtained using standard cholesterol solutions of 25-400mg/dl was determined as y= 0.0009x.Since the absorbance of the serum sample was determined as 0.28, how many mg/L is the amount of cholesterol in the sampleFrom a 5% normal saline (NaCl) solution, take 2 ml and dilute it 1:5. Take 5 ml of this solution and dilute it 1:10. A 2:100 dilution is made from this last solution. Determine: a) How many times less concentrated is the 2nd dilution than the original? b) What is the dilution factor in each step of the series and what is the final volume of each dilution? c) How many grams of NaCl will be in 1 ml of the last solution?a) A bottle is labeled Demerol (meperidine) 50 mg/cc. How many cubic centimeters are needed to give a 125 mg dose? b) Promazine HCL comes as 500 mg/10 ml. How many milliliters are needed to give 150 mg dose? c) Hyaluronidase comes as 150 U/cc. How many cubic centimeters for a 30 U dose?
- Reports have indicated that a metabolic byproduct of Vitamin B6 produces 4-pyridoxic acid.(1) This metabolite has fluorescent properties to could “cross-talk” with quinine (i.e., increase the fluorescent signal used for quinine and result in artificially high quinine values).(2) What techniques could we employ to separate 4-pyridoxic acid and quinine in the urine sample? This is a BSL-2 lab.A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working1) If you have 2.5g of hydrocortisone POWDER, how many grams of a 0.05% ointment can be compounded? 2) How many mL of a 0.45% saline solution can be made with 6750 mg of sodium chloride? 3) Your pharmacy receives an order to compound 120 g of a 1.25% cream. How many mg of drug are required to compound this order? 4) Is it possible to make 1200 mL of a 30% dextrose solution with only 75 g of dextrose? 5) You are asked to compound a 15% zinc oxide cream. How many grams of this cream can you make using 30,000mg of zinc oxide? 6) Using 45g of acetic acid, how many mL of a 20% acetic acid solution can be made? 7) Calculate the percent strength of a solution when 8 g of drug is dissolved in 400 mL of solution. 8) Calculate the percent strength of a solution when 2.5 g of drug is dissolved in 750 mL of solution. 9) Calculate the percent strength of a solution when 5000 mg of drug is dissolved in 500 mL of solution.
- You have an order for 1 gram of Cefazolin in D5W 100 ml. You have added 5 ml of sterile water to the 1 gram vial to reconstitute powder. However the recommended manufacturer’s diluent amount is 10 ml of sterile water for a final concentration of 100 mg/ml. How would reconstituting the vial with 5 mls affect the concentration and the final calculated dose?Caffeine is used in bilirubin assays to: 1) Stop the diazo reaction 2) Accelerate indirect bilirubin reaction 3) Accelerate direct bilirubin reaction 4) Precipitate proteins 5) Reduce sodium nitrite no references, just homeworkWhich of the following statements is correct about column chromatography? a) Resolution improves as column length increases b) Mobile phase is a porous solid material kept in the column with appropriate chemical characteristics c) Stationary phase is a buffered solution that percolates through the mobile phase d) Large proteins exit the column faster than tiny proteins
- A solution of flycine and a solution of albumin were prepared with the same concentration (g/L). One of the soutions turned light purple and the other dark purple for hte ninhydrin test. Which one would you expect to give the darker purple color? Explain.Absorbance is directly proportional to glucose concentration for both routine clinical glucose spectrophotometry methods: glucose oxidase method and the hexokinase method. For each one, name the final product measured in each test reaction, which is proportional to the sample glucose concentration. A.) glucose oxidase: B.) hexokinase:Please answer fast AFFINITY CHROMATOGRAPHY a) Explain the mechanism by which the CB column binds LDH. b) Discuss the reason by which the column is washed with Tris buffer after the sample has been applied. c) What is the method to recover LDH bound to the column.