What is “washing” in immunology? What types of interactions does washing interfere with? What molecules are used for effective “washing” in the immunology lab? b) Blocking is also a common feature of immunological assays. Why is blocking required?

Q: How would a baby benefit from a nursing mother receiving a vaccine for COVID-19? Make sure you…

A: A vaccine is a biological preparation that will provide and develop an active acquired immunity to a…

Q: What types of compounds in bacterial cells can serve as antigens?

A: Bacteria are prokaryotic cells, they are present in various shapes in nature like rod-shaped,…

Q: Which option is not an example of active immunization? Group of answer choices Injection of a mRNA…

A: Active Immunity  Antibodies develop in a person's own immune system after the body is exposed to an…

Q: All of the antibodies are shown with just one antigen binding site occupied. If a single antibody…

A: Answer- Each antibody have two binding sites for the foreign antigens. But more complex antibodies…

Q: What are the ordered steps of an ELISA protocol? A. Add primary antibody->wash-> Bind sample to a…

A: INTRODUCTION ELISA Enzyme Linked Immunosorbent Assay, used to detect antibodies in the blood.

Q: Illustrate the solid phase “sandwich” immunoassaY AND What does the “s” in HBs stand for? What other…

A: Introduction: The antigen-antibody reaction is an association between an antigen and the antibody…

Q: Would it be worse for the function of the antibody for it to lose the Fc region or a Fab region?…

A: The  N-terminal fragment square degree is called the beautiful space, and also the C-terminal…

Q: Describe the process of immunohistochemistry to detect a specific protein of interest.

A: or ImmunoHistoChemistry ( IHC), is an uncommon staining measure performed on new or frozen breast…

Q: Why is a blocking buffer needed while running the immunoblotting of the PDVF membrane, furthermore…

A: Immunoblotting, like ELISA, uses host antibodies to identify specific parasite antigens. Protein…

Q: What is the principle of Immunochromatographic system, and its uses? Long answer please

A: Answer: CHROMATOGRAPHY TECHNIQUE = It is the technique which is used to separate the components of a…

Q: How can the genome of a pathogen guide the vaccine designing ? How will the genome sequence…

A: The cellular mechanism in which the cell's internalization occurs and involved substances like…

Q: What is the name of protein subjected to IP? What is the importance of the pY blot? How many…

A: Immunoprecipitation or IP is a technology that precipitates a protein from the solution with the…

Q: EIAs are extremely sensitive diagnostic tools. Why,then, is the immunoblot (Western blot) procedure…

A: EIA stands for enzyme immunoassay, is a diagnostic test that can be divided into two types based on…

Q: What is the point to labeling a secondary antibody with a marker that can be visualized instead of…

A: Introduction: The antibodies are used to detect certain antigens in a technique called ELISA. In…

Q: e principle immunoturbidimetric C3 assay

A: Complement C3 is a blood test that determines how active a specific protein is. The complement…

Q: Which of the following is NOT a way that an antibody can destroy a bacterial cell? a.…

A: Ans :- option (D) is right

Q: Name and in brief describe the process by which immunological diversity is generated ?

A: Introduction  Antibodies can be induced by virtually all microorganisms. Antibodies must be diverse…

Q: How will you describe sample # 5 and sample # 2 in terms of immunological identity ? a. Partially…

A: It is a double immuno- diffusion technique.

Q: You are an energetic immunology student who has isolated protein X, which you believe is a new…

A: B cells or B lymphocytes are a type of white blood cell found in the body. It is an immune cell that…

Q: why do we have to do a western blot confirmatory testing after doing an ELISA screening test for…

A: The human immunodeficiency virus causes the chronic virus infection known as acquired…

Q: Which of the following represents the correct order of experimental steps for the Western Blot? A)…

A: Western blot is an analytical technique to identify the presence of a specific protein within a…

Q: Describe the two immunological diagnostic techniques that rely on a secondary antibody and explain…

A: Protein molecules known as antibodies or immunoglobulins are created by the body's immune cells.…

Q: Explain why IgG subclasses is most efficient at activating complement?

A: Ans: IgG is one of the class of antibody important in eliciting the immune response.

Q: For Western Blot detection of specific proteins, the secondary antibody directly binds to: A) The…

A: An antibody is a protein molecule generated in the living body by plasma membrane of B-cells, which…

Q: to immunotechnology, answer the following: Give ONE main difference between: Murine MABs and fully…

A: The capability of the body to induce immune response is called immunity. The foreign particle that…

Q: hy cannot freeze Human Normal Immunoglobulin injection?

A: Immunoglobulin , which is also known as gamma globulin, is a therapeutic preparation which is…

Q: Briefly Explain this statement "Immunomodulator Agents "-Please explain at your own words.

A: There are certain drugs  which can modulate the immune response are called Immunomodulator. They are…

Q: Digestion of antibodies with mercaptoethanol yields: Select one: 2 Fab,  Fc 2 Fab, and 1 Fc 4…

A: Antibodies are polypeptide chains produced in the body in response to an antigenic attack, also…

Q: What are steps in making an inactivated vaccine?

A: Vaccinations and Immunizations started in the 18th century, with the Smallpox Vaccination, created…

Q: Identify two immunological diagnostic techniques that rely on a secondary antibody, and explain how…

A: Serology is the scientific study of blood serum and other bodily fluids. In practical immunological…

Q: What is the function of Fab and Fc region on an antibody?

A: Given: What is the function of Fab and Fc region on an antibody?

Q: In the more common protocol for immunofluorescence detection of cellular proteins, an investigator…

A: Antibodies have Fc and Fab parts. The Fab part binds with the epitope of the antigen. The Fc…

Q: t Are The Uses Of Indirect Immuno Fluorescence?

A: The immunofluorescence technique, which used a fluorescence microscope to read the specific…

Q: hinge region Fc Fab kappa alpha chain Drag answer here Drag answer here. Drag answer here Drag…

A: The structure of the antibody is Y shaped with four polypeptide chains with two heavy and two light…

Q: You want to detect troponin in an immunoblot. Give an example of a potential primary rabbit antibody…

A: Immunoblotting is a sensitive and rapid assay to detect and characterize proteins. It works by…

Q: explain or describe the principle of immunoturbidimetric C3 Assay.

A: Immunoturbidimetric: A technique that calculates the absorbance of light from an example which is…

Q: 1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction…

A: Note: Since you have posted a question with multiple sub-parts, we will solve the first three…

Q: What are the two fluorescent compounds that can be used in antibody labelling?

A: Immunoassays make use of a wide variety of antibodies to detect and quantify antigens. In these kind…

Q: In your own words, explain why this procedure is referred to as an enzyme-linked immunosorbent…

A: ELISA is the most widely used test in serological diagnosis. It is well used for the detection of…

Q: What is the reason behind antibody diversity?

A: When our bodies are exposed to pathogens like bacteria and viruses, they produce a wide range of…

Q: Which of the following does not contribute to generating the diversity of antigen-binding…

A: The antigen is any agent able to induce an immune response in the human body. These are recognized…

Q: Outline the principle behind the following techniques:…

A: Immunofixation is an immunological technique that involves antigens and antibodies. Southern blot is…

Q: Why does the eliza with two antibodies incubate after adding the substrates?

A: ELISA are a type of immunoassay that are commonly used to quantify the specific target within a…

Q: In the following experiment, strain B mouse is subjected to sublethal irradiation and a bone marrow…

A: Innate and adaptive immune responses are characteristics of the immune system. The immune system's…

Q: Attenuated agents may induce immunity after a single dose may cause disease in immunocompromised…

A: Attenuated agents are live but weakened agents or pathogens