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- explain or describe the principle of immunoturbidimetric C3 Assay.1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge true/false 3) PBST is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8Can S-layer proteins be detected by immunolabelling when a capsule is present? How do you know? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/
- As a research scientist working on MS detail the possible strategic directions you could take to produce and purify large quantities of the human interferon β-1b. choose which strategy you think the team should take and why.Serum from individuals with high levels of antibody to SARS-CoV2 has been used to treat patients with severe COVID-19. What is ONE way (there are several) that passive immunization with the antibody to the virus could help these patients? HINT: think about what opsonization with antibody could do for the innate immune response.Outline the principle behind the following techniques: 1. Immunofixation 2. Southern blotting 3. Ion exchange chromatograph 4. SDS-PAGE.
- In relation to immunotechnology, answer the following: Give an example of murine Monocolonal Antibody produced by hybridoma technology?How would a lateral flow immunoassay using breast milk to test high levels of concentrations of IgG from a maternal mother whom was vaccinated to protect her child from RSV logistically be made? Specify the importance of making this a rapid investigative device and select the test and control antibody and whyyou chose them. Present a diagram if possible.In relation to immunotechnology, answer the following: explain the reason behind developing different forms of Monoclonal antibodies (chimeric MABs, humanized MABs, and fully human MABs)
- Why is a blocking buffer needed while running the immunoblotting of the PDVF membrane, furthermore why is skim mil powder often used in the buffers?2. https://doi.org/10.1186/s12868-022-00692-1 (link to research) a) In the immunohistochemistry section of the materials and methods section the authors wrote “The number of positive cells in hotspot areas in ten high power fields (HPFs) in areas of demyelination and plaques in the brain stem were counted using the image analysis software (Lecia Application Suite Version 4.12.0, Welzlar, Germany).” Why were they looking at demyelination areas for this study? b) In the effect of mitoxantrone on histopathological changes in the brain section of the results section the authors wrote “Active plaques revealed inflammatory cellular infiltrates with abundant macrophages stuffed with myelin debris, an evidence of ongoing myelin breakdown.” What does macrophages stuffed with myelin debris have to do with the study?In this chapter, we described co-immunoprecipitation as a method for identifying binding partners to a protein of interest. A simpler variation of this method can also be used to isolate proteins of low abundance in a complex mixture. Arrange the steps in sequential order to use this technique for this purpose. First step Last step Answer Bank The protein mixture is centrifuged, the supernatant is removed, and the pellet is washed. The antibody binds to the protein of interest. The antibody-protein complexes become insoluble. An antibody against the protein of interest is added to the mixture. Protein A beads are added to the mixture.