What's the amount of protein if the measurement at A280 = 1.120, dilution factor is 100 and total volume of extract is 45
Q: In 2-3 sentences, explain what system the authors are studying in this work. Describe what this syst...
A: Enzymes play a vital role in biology since they reduce the energetic barrier separating substrates f...
Q: ...::: What if there is too much amino acids in your body, what will happen to your body?
A: If there are many too many amino acids present in the body then there are serious harmful effects of...
Q: Circle and label the hemiacetal functional group and the acetal functional group in each of the foll...
A: Carbohydrate is a macronutrient that the body uses to generate energy and form complexes that are ne...
Q: 6 After running an Ion exchange chromotograpraphy column and using cation exchange, will the elution...
A: Ion exchange chromatography is used to separate molecules based on the affinity of the target molecu...
Q: In the first step of the citric acid cycle, an acetyl group from acetyl CoA reacts with oxaloacetate...
A: Citric acid cycle is one of the steps in aerobic oxidation of glucose. It occurs in mitochondrial ma...
Q: 64 kDa, Tetramer (a B)2 32 kDa, Dimer αβ 16 kDa, Monomer or B a Elution Volume (mL) Absorban ce at 2...
A: Hemoglobin is functionally a tetrameric complex of 64kDa where two α and two β subunit form Heterote...
Q: 10. Which of the following statement(s) is/ are true for the molecule below? (1) It can be found on ...
A: Structure of the monosaccharides can be represented using the Fischer projection or Haworth projecti...
Q: Determine the statement that is not TRUE about Dietary Reference Intake recommendations. DRI recomme...
A: The statement which is not true is:
Q: 16. What decides and allows amino acid residues are phosphorylated or dephosphorylated? 17. åre proc...
A: Phosphorylation of amino acids in the protein molecules plays an important role in signaling pathway...
Q: Describe the biochemical role of coenzyme Q10 and how supplementation may impact metabolism. Be sure...
A: Coenzyme Q is an important component of oxidative phosphorylation process which takes place in mitoc...
Q: Draw the tetrapeptide Met-Ile-Lys-Glu at a ph of 7?
A: The pKa values of amino acid side chains play an important role in determining the pH-dependent net ...
Q: 8. Increasing the number of these molecules in the cell membrane would increase the permeability of ...
A: Given are different biochemistry related statements and they tell about different molecules of it.
Q: Evaluating: Evaluate the following statements and determine which of them is FALSE about “agarose" *...
A:
Q: Directions: Supply the missing information. Amino Acid Sequences of in Cytochrome-c ANIMAL AMINO ACI...
A: Cytochrome C is a mitochondrial protein that is found in the mitochondria. It is highly water solubl...
Q: An H+ ion is smaller than an H2O molecule, and a glycerol molecule, a three-carbon alcohol, is much ...
A: Cell transportation can be outlined as a phenomenon in which there occurs movement of material acros...
Q: Review Figures 8.12 and 8.13. In cells, the primers forDNA synthesis are short strands of RNA, so ea...
A: A primer molecule is a short strand of RNA. During replication, the complementary nucleotides are ad...
Q: (a) Propose a biosynthetic pathway to the secondary metabolite, B. OH O H. HO Metabolite B
A: The given metabolite structure is derivative of Salicylaldehyde derivative and synthesized by plant ...
Q: What is the difference between obligate and non-obligate chain terminating nucleotides? How were sof...
A: Chain terminating nucleotides are nucleotides which terminates the RNA synthesis, that is also call...
Q: Classify amino acids according to their functional groups.
A: Amino acids are chemical molecules with amino and carboxylate functional groups, as well as a side c...
Q: IV. Write on the first column the monosaccharide unit(s) that is present in the given disaccharide. ...
A: Carbohydrates, abbreviated as carbohydrates, are sugar molecules. Saccharides are another name for s...
Q: What are the important considerations that you have to remember in antibody screening?
A: Since you have asked multiple question, we will solve the first question for you. If you want any sp...
Q: With reference to Table 2, what was the total extract volume in mL? Enter a number (no decimal place...
A: In cells, there are thousands of distinct proteins. Extraction and purification of these substances ...
Q: Are all the proteins separated properly using SDS-PAGE? Why or why not?
A: Proteins are massive biomolecules and macromolecules made up of one or more long chains of amino aci...
Q: Match the descriptions and compounds with the terms competitive, uncompetitive, and noncompetitive i...
A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation energy...
Q: n comparison to glycogen, fats can provide more energy for the body per carbon atom. Why? 1. Fat...
A: Fats belong to a group known as lipids. It is characterized by a greasy feel and they are insoluble ...
Q: What test(s) could you perform to test your hypothesis about the greenish precipitate. (Centrifuge ...
A: Centrifugation is the separation process by which the substances get separated on the basis of densi...
Q: 8. "Salting-out" is a process A. to represent the certain molecules are ionized in a solution B. whe...
A: Salt is a molecule composed of positive and negative ions when dissolved in water. Salt molecules ar...
Q: Topic: Determination of Protein Concentration by Spectrophotometry: The Standard Curve The use o...
A: Beer lamberts law is the basis of spectrophotometry. The principle of spectroscopy is based on the a...
Q: What is the order of the electron transport chain starting from FADH2 and ending with Oxygen
A: The energy released during the oxidation of glucose to carbon dioxide generates NADH and FADH2. The...
Q: Explain how does Ohm’s law relates to our Core Concept of flow down gradients in physiology
A: According to ohm's law, the amount of current flow or potential difference between two points is dir...
Q: la: Several factors (including agarose gel concentration, time and current) affect migration of DNA ...
A: Agarose gel electrophoresis is the technique that is used for separation of DNA molecules based on t...
Q: Draw the tetrapeptide Met-Ile-Lys-Glu at a ph of 12?
A: The pKa values of amino acid side chains play an important role in determining the pH-dependent char...
Q: What are reducing sugars? Why is sucrose non-reducing while lactose also a dissacharide, is reducing...
A: Carbohydrates are biomolecules containing carbon, hydrogen, and oxygen. Carbohydrates are classified...
Q: Evaluating: If a DNA strand reads AATGCTCCAGT, what would the complementary strand read: * O GGCAATC...
A:
Q: Hello, please answer the questions below about Vitamin C. Name of Vitamin: Vitamin C Classification...
A: Introduction: Vitamins are natural substances found in both plants and animals and it is considered ...
Q: 5. If enzymes are truly specific, they will catalyze the reaction of one substrate only. Predict whe...
A: Hi! Since you have posted multiple questions and have not mentioned which to answer, we will answer ...
Q: a) Create a bacterial growth curve with log values of CFU/ml on the Y-axis andthe time
A: Bacterial growth curve is a graphical representation of number of live bacterial cells over the time...
Q: Consider the biochemical pathway below, where A, B, and C are substrates and products and E1 and E2 ...
A: Enzymes are proteinaceous in nature. Almost all enzymes are proteins, there are some nucleic acids t...
Q: What is plant biochemistry?
A: Introduction - Plant Biochemistry is the study of different chemical reactions in the plant such as...
Q: Which of the following statements are true about stacking and separating gels? a. The holes is t...
A: In the SDS-PAGE technique, stacking gel is a low concentration polyacrylamide gel that is placed on ...
Q: Hello good day, I am having a problem answering this question and I need your help on this. Hoping f...
A: The G6PD gene codes for glucose-6-phosphate dehydrogenase, which is a type of enzyme. The correct me...
Q: If the nucleotide at position 23 in thw first strand of DNA ia changed to "A", what effect would thi...
A: Understanding changes in DNA is very important to study presence of mutations and their phenotype ef...
Q: What is vaccine? Please be sure to include what molecules are present and the biological mechanism b...
A: Immunity is described as a complicated biological system that has the ability to recognise and accep...
Q: Explain why the maximum initial reaction rate cannot be reached at low substrate concentrations
A: Multiple questions asked. I will answer the first question, as allowed by guidelines. Please repost ...
Q: My PDB code: 3GRS residue point: 66 (LYS) mutation: VAL
A: 3GRD.PDB is the crystallographic structure of GLUTATHIONE REDUCTASE solved at1.54 ANGSTROMS resoluti...
Q: With respect to the above molecular outline, what chemical properties would you expect for the (a) h...
A: A fatty acid is a carboxylic acid with an aliphatic chain. Soap is sodium salt of fatty acid.
Q: TRUE OR FALSE Palmitic oil is (soluble) to hot ethanol and chloroform.
A: Hi. Thank you for the question. The second question looks incomplete. Please provide the details abo...
Q: Density lipoprotein,reduces the risk of atherosclerosis by clearing out fatty plaques in the blood v...
A: There is unequivocal evidence of a negative relationship between plasma high-density lipoprotein (HD...
Q: How are inhibiting agents different from those that denature an enzyme? Use the paperclip button bel...
A: Enzymes are proteins which increase the rate of biochemical reactions. All proteins become active on...
Q: The role of a subunit of F0 (2 choices are correct)
A: ATP synthase is a mitochondrial enzyme machinery which is located in the inner membrane of mitochond...
What's the amount of protein if the measurement at A280 = 1.120, dilution factor is 100 and total volume of extract is 45
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- [protein] volume [protein] [protein] in to Sample Lysate in "gel- load 5 in 20 protein in Name volume assay* lysate ready" ul sample protein 3.9 2 µl Hg/ml Tilapia 19.9 10 μι Hg/ml 18.2 2 µl Hg/ml Cod >20 14 10 μι Hg/ml 9.4 15 2 µl ug/ml Sole >20 6. 10 μι ug/ml > >Calculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.197, taking into account the dilution factor 100 per and total volume of extract 140mlDiscuss the functions and components of Biuret dye in protein content determination. Identify two other methods of protein determination and differentiate them from Biuret method. Why is BSA used as standard for protein content determination? Can other proteins be used instead?
- Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.From this standard curve and chart below, does the separation of molecules in the mixture appear successful from the gel filtration? Is there a clearlydefined separation between molecules? Explain your conclusions. Parameters required for calculation of coefficient (Kd) for unknown protein Volume eluted (mL) Which variable does this volume represent in the equation for Kd? Fraction with maximal DNP-Aspartate detected 36 Vt Fraction with maximal Protein detected 24 Ve Fraction with maximal Blue dextran detected 6 VoA gel filtration column was calibrated by measuring elution volumes for two proteins of known mass: glutamate dehydrogenase, 290 kDa with elution volume 20.8 mL; serum albumin, 37 kDa with elution volume 42.5 mL Use these values to determine the equation of the straight line graph that relates log molar mass to elution volume. The elution volume of an unknown protein was 32.2 mL. Use the equation just determined above to estimate the molar mass of the protein in kDa units. Don't round your answer until the end to ensure your answer is within the accepted range. Round your answer to the nearest whole number (e.g. If you calculated 27.52, input your answer as 28).
- AE, 62, Male Wt: 75 kg, Ht: 150 cm Orders: Penicillin G Potassium 10,000,000 Units Sterile water for injection qs Make a 250,000 U/mL solution. The package insert states If Penicillin G Potassium 10,000,000 Units is reconstituted with 44 mL sterile water, a solution of 200,000 U per mL will be obtained. What volume of sterile water would you use to reconstitute the penicillin G potassium in order to make the ordered solution?The order is for Moxam 5 gm IM every 8 hours. On hand is 10 gram of Moxam with the following directions. Add 8 mL of diluent to yield 10mL of reconstituted solution. How many mL will you give?A mixture of proteins contains Pepsinogen (35 kDa), Fumarase (49 kDa), Transferrin (80 kDa) and Thyroglobulin (340 kDa). Rank these proteins based on the order of their elution from a gel filtration column (1 being the first one to elute and 4 as the last one). Throglobulin Pepsinogen Fumarase Transferrin
- Wh are doing this procedures can you explain? (ex. heating or adding chemicals etc.) 1) Purification of Vitelline from Egg Yolk-Experimental ProcedureMeasure the volume of 3 egg yolks and mix by adding an equal volume of NaCl solution.Extract the mixture with 3-fold volumes of ether and separate the aqueous phase.Do the same procedure 3 times.Mix the sample with water and rinse.It is expected for the protein to collapse.Some more water is added in order to check whether the collapse occurs completely.The sample is centrifuged and the precipitate is dried. 2) Purification of Plasma Albumin and Fibrinogen-Experimental ProcedureAdd ammonium sulfate to 10 ml plasma up to 25% saturation.Separate the collapsed fibrinogen by centrifuge.Increase the saturation level to 33% by adding (NH4) 2SO4.Separate the globulin by centrifugation.Separate prodoglobulins by increasing the saturation level to 46%.Increase the saturation level up to 64% to precipitate albumin.Separate the albumin by centrifuge…Calculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.636, taking into account the dilution factor as per question 6 (100ul) and the total volume of extract as per question 4 (140ml). Give your answer as a number with no decimal places.A series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was subjected to a Bradford assay. The increase in absorbance at 595 nm relative to the concentration of protein was plotted as shown below. Save 1.0- 0.8- 0.6- 0.4- 0.2- 0.04 0.0 0.5 1.0 2.0 2.5 1.5 BSA concentration (mg/mL) a) Using the graph, calculate the original concentration of protein present in a mixture of haemoglobin and methylene blue (diluted 1:50), which gave an absorbance of 0.20 at a wavelength of 595 nm using the Bradford assay. Show all workings. Absorbance (O.D.)