You are running an ELISA to test three patients for COVID-19.The results of the test are shown below (and in the PDF).What statement is TRUE based on the results shown?
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- The following antibody screen was performed on a 65 year old male who was hospitalized for a fractured hip. 2. Indirect Antibody Screen IS 37°С АНG Check Cells Patient serum + Screen cell I 1+ 2+ Patient serum + Screen Cell II 1+ 2+ Patient serum + Screen Cell III 1+ 2+ Patient Serum + Patient Cells 1+ 1+ 2+ a. What is the most likely explanation of these results? b. And what should the technologists do next to resolve for any underlying alloantibodies? 3. Upon completing of a patient type and screen, it was found the patient has a positive auto control. A DAT on the patient reveals the following results. This patient is not on penicillin. Direct Antiglobulin Test Polyspecific AHG 2+ Anti-IgG 2+ Anti-C3d Patient Cells 2+ Check Cells NA NA NA а. What is coating the patient cells? b. What could possibly cause these results?A patient is O positive and his serum is agglutinating all screening and panel reagent cells at room temperature. His autocontrol is positive at room temperature also. The patient may have an antibody to a high incidence antigen. One possibility is that he may have a clinically insignificant autoanti-I. How could you prove that the patient has an anti-I antibody?A physician is typing the blood specimen belonging to an eighteen-year-old female to receive emergency blood transfusions due to an unfortunate accident. The following are the results: Anti-A Anti-B Anti-D A1 Cells B Cells 4+ - - - 4+ Due to the initial negative Anti-D result, the medical technologist decided to perform an indirect antiglobulin test. The following are the results: Anti-D Control Positive Neg Using the IAT results, should the patient be considered Rh positive or Rh negative? Justify.
- A thyroid peroxidase antibody test was performed on a 45 year old man. The dilution sequence was 25 microliters serum to 100 microliters of diluent in tube 1. Then 25 microliters from tube 1 was added to 25 microliters of diluent in tube 2. Then 25 microliters from tube 2 was added to 50 microliters of diluent in tube 3. What is the dilution factor of tube 1. What is the dilution factor in just tube 2. What is the dilution factor in just tube 3. What is the overall dilution factor in tube 3.You are interested in performing indirect immunofluorescence light microscopy to observe the localization of the catalase enzyme in the cultured HeLa cells, obtained historically from the cervical tumor of Henrietta Lacks. You were going through the lab stock and found a few primary and secondary antibodies. Which of the following secondary antibody can you use in your experiments? O All of the mentioned antibodies can be used in the experiment Goat anti-human antibody conjugated to 10 nm gold Goat-anti-human catalase conjugated to 10 nm gold O Human anti-catalase antibody conjugated to fluorescent rhodamine Goat anti-human antibody conjugated to fluorescent rhodamineEnzyme immunoassay tests are used to screen blood specimens for the presence of antibodies to HIV, the virus that causes AIDS. Antibodies indicate the presence of the virus. The test is quite accurate but is not always correct. Approximate probabilities of positive and negative test results when the blood tested does and does not actually contain antibodies to HIV are given in the table. Test Result Positive Negative Antibodies present 0.9985 0.0015 Antibodies absent 0.0060 0.9940 Suppose that 1%1% of a large population carries antibodies to HIV in their blood. Probabilities from trials with 2897 people known to be free of HIV antibodies and 673 people known to be infected, reported in J. Richard George, "Alternative specimen sources: methods for confirming positives," 1998 Conference on the Laboratory Science of HIV, found online at the Centers for Disease Control and Prevention, www.cdc.gov. (b) What is the probability that the test is positive for a…
- Which of the following components can be used in an ELISA? I. Polyclonal antibodies II. Monoclonal antibodies III. Protein A immobilized on agarose beads Select one: A. II and III only B. I only C. Il only D. I, II and III E. I and II only O O O O ORheumatoid Factor RF Latex Test RF is a rapid latex agglutination test kit for the detection of rheumatoid factor (RF) in human serum. RF is found in sera of patients with rheumatoid arthritis and is believed to be IgM antibodies directed against the patients own immunoglobulin G. Principle: The RF latex particles are coated with specially purified human gamma globulin. When the latex suspension is mixed with serum containing elevated RF levels on a slide, clear agglutination is seen within 2 minutes. Questions 1. Is Rheumatoid Arthritis an autoimmune disease? 2. Differentiate the different types of Immunoglobulins 3. Differentiate Rheumatoid Arthritis from Gouty ArthritisWhat are the ordered steps of an ELISA protocol? A. Add primary antibody->wash-> Bind sample to a surface ->Add substrate ->Add secondary antibody-enzyme conjugate ->wash B. Bind sample to support -> Add substrate -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash C. Bind sample to a surface -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash -> Add substrate D. Add secondary antibody-enzyme conjugate -> wash -> Add primary antibody -> wash -> Add substrate -> Bind sample to surface
- Following is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…An MLS has completed an antibody panel using PEG. She cannot exclude anti-Fya, or anti-E. Anti-Kpª and anti-Ja cannot be excluded either but they are low incidence antigens and the MLS is not concerned with those at this time. What would be the next logical step to aid in antibody identification? a. Run the panel again using ficin treated cells b. Run the panel again using 22% albumin for enhancement c. Pay special attention to the strength of the agglutination reactions d. Run the panel again but increase the 37 degree incubation time to one hourPatient A, who is a recipient of USA-based poly X8650-CA vaccine for its autoimmune allergic rhinitis often visits the doctor twice a month to complain for “idiopathic colds”. Meanwhile Patient B, who is currently taking DOH pilot-used trial 1 mono E2128-PH vaccine for the same condition do not have to visit its doctor. Based on the principles of the antibody production, can you explain why patient A visits more often?