Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 15, Problem 1CA
Summary Introduction

To determine:

Use of urease for Helicobacter pylori.

Introduction:

Urease is an enzyme that helps in the hydrolysis of urea, leading to the formation of ammonia and carbon dioxide. Urease rich seeds are soybeans, plant seeds, and jack beans. It also found in various organisms and is also secreted by intestinal microbes.

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Flasks A and B contain broth, and are inoculated with an identical number of bacteria that are facultative anaerobes. Flask A contains a triglyceride lipid as the sole nutrient source, and Flask B contains a monosaccharide called fructose as the sole nutrient source. Both flasks are incubated at 30°C with aeration (oxygen is added to the broth). Assume the bacteria are capable of catabolism of both fructose and triglycerides. Mark all the correct statements pertaining to these cultures. A. Under anaerobic conditions, cells in flask A will produce more ATP B. Cells from flask B will produce more carbon dioxide C. Cells from flask B will produce more ATP D. Flask A will have the greater cell mass E. Cells from flask A will produce more ATP F. Cells from flask A will produce more carbon dioxide G. Flask B will have the greater cell mass H. Under anaerobic conditions, cells in flask B will produce more ATP
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