Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 17, Problem 9PDQ
What are the advantages of using a restriction enzyme whose recognition site is relatively rare? When would you use such enzymes?
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What are the advantages of using a restriction enzyme whose recognitionsite is relatively rare? When would you use such enzymes?
What does it mean if a restriction enzyme produces ‘sticky’ or ‘blunt’ ends? Does the restriction enzyme HaeIII produce “sticky” ends or “blunt” ends?
Describe two features that are characteristic of the action of restriction endonucleases. How do these features make restriction endonucleases useful to genetic engineers?
Chapter 17 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 17 -
CASE STUDY |Should we worry about recombinant DNA...Ch. 17 - Prob. 2CSCh. 17 - Prob. 3CSCh. 17 -
HOW DO WE KNOW?
1. In this chapter we focused on...Ch. 17 - Prob. 2PDQCh. 17 - What roles do restriction enzymes, vectors, and...Ch. 17 - Prob. 4PDQCh. 17 - Prob. 5PDQCh. 17 - Prob. 6PDQCh. 17 - Prob. 7PDQ
Ch. 17 - List the advantages and disadvantages of using...Ch. 17 - What are the advantages of using a restriction...Ch. 17 - The introduction of genes into plants is a common...Ch. 17 - Prob. 11PDQCh. 17 - Prob. 12PDQCh. 17 - Prob. 13PDQCh. 17 - What advantages do cDNA libraries provide over...Ch. 17 - Prob. 15PDQCh. 17 -
16. List the steps involved in screening a...Ch. 17 -
17. In a typical PCR reaction, describe what is...Ch. 17 -
18. We usually think of enzymes as being most...Ch. 17 - How are dideoxynucleotides (ddNTPs) structurally...Ch. 17 - Prob. 20PDQCh. 17 - One complication of making a transgenic animal is...Ch. 17 -
22. When disrupting a mouse gene by knockout, why...Ch. 17 - Prob. 23PDQ
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- For a restriction enzyme that recognizes the restriction site GGCC, Which of the following statements is/are true?arrow_forwardWhat is a restriction enzyme? What structure does it recognize?What type of chemical bond does it cleave? Be as specific aspossible.arrow_forwardHow are restriction endonuclease enzymes named? Write examples.arrow_forward
- What is the function of a restriction enzyme?arrow_forwardIn an attempt to clone a gene into the TOL plasmid, it was observed that the restriction product of the gene of interest were blunt ended, thereby reducing the ligation efficiency of this gene and the TOL plasmid. Elucidate two methods that could be employed to increase the ligation efficiency of your restriction products.arrow_forwardHow to determine the location of restriction sites for different enzyme ?arrow_forward
- Restriction sites are palindromic; that is, they read the same in the5' to 3' direction on each strand of DNA. What is the advantage ofhaving restriction sites organized this way?arrow_forwardWhat normal role do restriction enzymes play in bacteria? How do bacteria protect their own DNA from the action of restriction enzymes?arrow_forwardWhich of the following can be termed as a restriction modification system?a) Restriction endonuclease + methylaseb) DNA ligase + methylasec) Restriction endonuclease + acetylased) DNA ligase + acetylasearrow_forward
- RFLP's result from mutations in: 1) genes which specify restriction enzymes 2) restriction enzyme recognition sites 3) genes which specify plasmids 4) monomorphic genesarrow_forwardRefer to the diagram of pUC18 (Fig.) to determine which restriction enzymes you could use to insert a gene that would interfere with production of β-galactosidase by the host cell.arrow_forwardIf restriction endonucleases are produced by bacteria within a host, why don’t these enzymes chew up the genomic DNA of their host? What is the role of DNA methyltransferase in this? Indicate the answerarrow_forward
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