Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 18, Problem 3AL
You are developing a new vaccine for a pathogen. You want your vaccine to recognize specific cell-surface proteins. Explain how you will use genome analysis to identify potential protein targets. What functional genomics approach will you use to determine which of these proteins is produced when the pathogen is in its host?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
To understand the genes responsible for growth and infectivity in a disease-causing bacterial strain, you perform chemical mutagenesis on a culture of these bacteria. In the course of your investigation into the properties of the resulting mutants, you identify a set of mutant bacteria that is still viable but their virulence is significantly impaired. How might these mutants be useful for vaccine development?
During the adenoviral SARS-CoV2 vaccine development process, the plasmid encoding the Spike protein is generated. Elaborate on the steps you would follow to generate a recombinant plasmid encoding the Spike protein.
You have constructed a cDNA library in a phagemid vector.Describe how you would screen the library for a particulargene of interest. Describe methods using oligonucleotideand antibody probes.
Chapter 18 Solutions
Prescott's Microbiology
Ch. 18.1 - MICRO INQUIRY What is the function of the 3-OH...Ch. 18.1 - MICRO INQUIRY Why is it important that identical...Ch. 18.2 - MICRO INQUIRY Which step (or steps) in this...Ch. 18.2 - Retrieve, Infer, Apply Why is the Sanger technique...Ch. 18.2 - Retrieve, Infer, Apply Explain the difference...Ch. 18.2 - Retrieve, Infer, Apply Why does reversible chain...Ch. 18.2 - Prob. 4CCCh. 18.2 - Retrieve, Infer, Apply Suggest a medical and an...Ch. 18.3 - Retrieve, Infer, Apply NGS techniques are...Ch. 18.3 - Retrieve, Infer, Apply Examine figure 18.8. How...
Ch. 18.4 - Prob. 1MICh. 18.4 - Prob. 1CCCh. 18.4 - Prob. 2CCCh. 18.4 - Prob. 3CCCh. 18.5 - Figure 18.12 Metabolic Pathways and Transport...Ch. 18.5 - Prob. 2MICh. 18.5 - Prob. 3MICh. 18.5 - Prob. 1CCCh. 18.5 - Retrieve, Infer, Apply How might the following...Ch. 18.5 - Retrieve, Infer, Apply Compare and contrast...Ch. 18.5 - Retrieve, Infer, Apply Why does two-dimensional...Ch. 18.5 - Retrieve, Infer, Apply What is the difference...Ch. 18.5 - Retrieve, Infer, Apply Describe a ChIP-Seq...Ch. 18.7 - Prob. 1MICh. 18.7 - Retrieve, Infer, Apply Cite an infectious disease...Ch. 18.7 - Prob. 2CCCh. 18.7 - Prob. 3CCCh. 18 - Prob. 1RCCh. 18 - Prob. 2RCCh. 18 - Prob. 3RCCh. 18 - Prob. 4RCCh. 18 - Prob. 5RCCh. 18 - Prob. 1ALCh. 18 - Prob. 2ALCh. 18 - You are developing a new vaccine for a pathogen....Ch. 18 - Prob. 4AL
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Moderna and Pfizer vaccines for COVID-19 use mRNA to the Spike protein combined with lipids. Which of the following is/are advantages of this type of vaccine compared to an inactivated virus vaccine? Select ALL correct answers. a) The vaccine is more stable for transport. b) The vaccine does not contain the virus so it cannot give you COVID-19. c) The vaccine can be rapidly produced in a slightly different form to protect from variants of SARS-CoV-2. d) The vaccine does not need a booster.arrow_forwardWhat is a recombinant DNA vaccine?Give two examples.arrow_forwardYou are a graduate student working to construct a single gene knockout library of Leptospiria kirschneri, one the causative agents of leptospirosis. You are looking for single gene mutants which disrupt the bacterium’s spirillum shape to determine what role this rare cellular morphology may play in disease development and progression. Using an appropriate donor strain, you introduce the plasmid shown into L. kirschneri. L. kirschneri is not able to replicate the plasmid. The repeat regions are denoted on the plasmid map as vertical black lines, the transposase is denoted as tnp, and kanamycin kinase is denoted as aph. The larger of the two regions is transposed. Following selection and counter-selection, you isolate several non-spirillum colonies, which you use to infect juvenile piglets. Most of the infected piglets develop leptospirosis. Isolating L. kirschneri from these animals reveals that it has regained its spirillum morphology. What is a likely explanation for this reversion of…arrow_forward
- You want to product human immunoglobulin G using a recombinant Escherichia coli system. Design the whole procedure and strategy by yourself.arrow_forwardThe Pfizer Covid-19 vaccine is one of the vaccines currently being rolled out for mass vaccination in South Africa to protect the population against SARS CoV-2, and the active compound in this vaccine is an mRNA molecule. a) The starting material for productio of this vaccine is a copy of the coronavirus spike protein gene cloned into a ppasmid. List the main experimental steps in the vaccine production process, to go from a plamid to an mRNA molecule. b) Which molecular processes in the cell will allow an injected strand of mRNA to produce anti-Covid immunity in the vaccine recipient?arrow_forwardBiochemistry techniques: a) Name and briefly describe the ELISA assay with greater sensitivity for detection of the viral infection. Focus on antigens and antibodies. Justify your reasoning. B) Consider a mixture of four proteins with various molecular weights. A histone molecule weighs 15 kDa, a p53 molecule weighs 53 kDa, an actin molecule weighs 42 kDa, and an IgG molecule weighs 150 kDa, Arrange the molecules in order of their elution from a gel filtration column. Elutes first (A) to elute last (D) arrangement below: A) B) C) D) Explain your reasoning . Instructor C). What characteristics determine the position of a protein at the end of isoelectric focusing of 2D SDS- PAGE?arrow_forward
- In Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.arrow_forwardProtein vaccines include only the parts (i.e. a protein) of a virus that best stimulate your immune system. This type of COVID-19 vaccine contains harmless S proteins. Once your immune system recognizes the S proteins, it creates antibodies and defensive white blood cells. If you later become infected with the COVID-19 virus, the antibodies will fight the virus. Assume that you work in a project for development of such vaccine against COVID-19. The vaccine contains S Protein and your job includes isolation of pure S Protein from the virus determination of charge on the protein (or pI) determination of molecular mass revealing three dimensional (3-D) structure of the protein How would you determine the pI of the protein?arrow_forwardProtein vaccines include only the parts (i.e. a protein) of a virus that best stimulate your immune system. This type of COVID-19 vaccine contains harmless S proteins. Once your immune system recognizes the S proteins, it creates antibodies and defensive white blood cells. If you later become infected with the COVID-19 virus, the antibodies will fight the virus. Assume that you work in a project for development of such vaccine against COVID-19. The vaccine contains S Protein and your job includes isolation of pure S Protein from the virus determination of charge on the protein (or pI) determination of molecular mass revealing three dimensional (3-D) structure of the protein How would you isolate (purify) the protein; suggest different techniques and explain the techniques. How would you determine the pI of the protein? Suggest techniques for determination of molecular mass. How would you analyze the structure of the protein?arrow_forward
- In recombinant DNA technology, vectors are used to transfer a gene of interest in the host cells. Mention any three features of vectors that are most suitable for this purpose.arrow_forwardProtein vaccines include only the parts (i.e. a protein) of a virus that best stimulate your immune system. This type of COVID-19 vaccine contains harmless S proteins. Once your immune system recognizes the S proteins, it creates antibodies and defensive white blood cells. If you later become infected with the COVID-19 virus, the antibodies will fight the virus. Assume that you work in a project for development of such vaccine against COVID-19. The vaccine contains S Protein and your job includes isolation of pure S Protein from the virus determination of charge on the protein (or pI) determination of molecular mass revealing three dimensional (3-D) structure of the protein Suggest techniques for determination of molecular mass. How would you analyze the structure of the protein?arrow_forwardMany ELISAs that are used to identify the presence of antibody to viruses have a 3rd control (along with negative and positive). In between the rows of wells coated with antigen, are rows of cells coated with tissue culture used to grow the virus. In other words, patient serum is added to wells that contain antigen along with wells that contain tissue culture. Why do you think this might be necessary?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License