Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 20, Problem 4CT
Why is turbidity not an accurate measurement of viable bacteria in a culture?
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In a bacterial culture on an agar plate, what is a zone of inhibition?
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An area where the the agar becomes contaminated with mold
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Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
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- After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?arrow_forwardWhy do we use turbidity as a method to measure bacterial growth? How does it work? How do you interpret the theoretical and actual results of turbidity measurements? Can you think of advantages and disadvantages to using this type of method?arrow_forwardOf what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?arrow_forward
- Why is it critical to be able to determine the overall number of bacteria present in a food sample?arrow_forwardA broth culture is initially inoculated with a single bacterial cell. For the first hour the bacteria has a generation time of 30 minutes, for the second hour the generation time has been reduced to 15 minutes, and for the third hour the generation time has been reduced to 10 minutes. At the end of the third hour how many bacteria are present in the broth culture?arrow_forwardWhat is the description of the growth and growth pattern of the microorganisms in the pour plate method?Type of Growth: Surface or Sub-surface?What is the typical growth pattern of inocula?arrow_forward
- There are various parameters to assess microbial growth. What are some microbial growth curve characteristics? Can you explain how microbiologists measure specifically bacteria growth?arrow_forwardwhy is it important that a small amount of inoculum be used in preparation of an agar slant?arrow_forwardwhat is the physical characteristics of this streak plate? Look at a single colony on a streak plate and look for special physical characteristics such as: motility, possible presence of endospores and/or capsule, culture color?arrow_forward
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