Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 3.L2, Problem 3CT
Summary Introduction
To analyze:
- The kind of medium to selectively grow marine bacterium.
- The kind of medium to bacteria of the human stomach.
- The ability of bacteria to grow on bile-containing medium.
Introduction:
Culturing microorganisms out of their natural environments in pure form in the laboratory has been made possible only with the development of artificial medium or media. The role of media in the culture, identification, and mass production of microbes is remarkable. Selective media can be prepared by providing the specific growth environment for a particular group of organisms.
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). Consider a culture medium on which only gram-positive organisms such as Staphylococcus aureus colonies can grow due to an elevated NaCl level. A yellow halo surrounds the growth, indicating the bacterium fermented sugar in the medium, decreasing the pH as a result and changing the color of a pH indicator chemical. This type of medium would be referred to as a(n):
A) Selective medium.
B) Differential medium.
C) Enrichment culture.
D) Selective and differential medium.
E) Differential and enrichment culture.
1a.) How pH of the culture medium affects the cell culture? Please give examples of low pH and high-pH culture conditions.
1b.) Describe the difference between defined and undefined cell culture media. What are the advantages and disadvantages of both of them?
a. Identify the cell shape, cell arrangement, and Gram reaction of the Unknown microbe in the image below.
b. Suppose the corresponding Gram negative control were purple. Explain what step(s) of the Gram stain you would adjust and how you would adjust them to ensure the accuracy of your Unknown results
Chapter 3 Solutions
Foundations in Microbiology
Ch. 3.1 - Explain what unique characteristics of...Ch. 3.1 - Briefly outline the processes and purposes of the...Ch. 3.1 - Name the notable features of microorganisms that...Ch. 3.1 - In one sentence each, define what is involved in...Ch. 3.2 - Prob. 3ELOCh. 3.2 - Prob. 4ELOCh. 3.2 - Prob. 5ELOCh. 3.2 - Prob. 6ELOCh. 3.2 - Prob. 7ELOCh. 3.2 - Prob. 8ELO
Ch. 3.2 - Prob. 3CYPCh. 3.2 - Prob. 4CYPCh. 3.2 - Prob. 5CYPCh. 3.2 - Prob. 6CYPCh. 3.2 - Prob. 7CYPCh. 3.2 - Prob. 8CYPCh. 3.2 - Prob. 9CYPCh. 3.2 - Compare the way that the image is formed in the...Ch. 3.3 - Prob. 9ELOCh. 3.3 - Define dyes and describe the basic chemistry...Ch. 3.3 - Prob. 11ELOCh. 3.3 - Distinguish between simple, differential, and...Ch. 3.3 - Describe the process of Gram staining and how its...Ch. 3.3 - Prob. 11CYPCh. 3.3 - Explain what happens in positive staining to cause...Ch. 3.3 - Prob. 13CYPCh. 3.3 - For a stain to be considered differential, what...Ch. 3.3 - Prob. 15CYPCh. 3.4 - Prob. 14ELOCh. 3.4 - Prob. 15ELOCh. 3.4 - Explain what an isolated colony is and indicate...Ch. 3.4 - Differentiate between a pure culture, subculture,...Ch. 3.4 - What kinds of data are collected during...Ch. 3.4 - Prob. 19ELOCh. 3.4 - Prob. 16CYPCh. 3.4 - Prob. 17CYPCh. 3.4 - Prob. 18CYPCh. 3.4 - Compare and contrast three common laboratory...Ch. 3.4 - Describe how an isolated colony forms.Ch. 3.4 - Prob. 21CYPCh. 3.5 - Prob. 20ELOCh. 3.5 - Name the three general categories of media, based...Ch. 3.5 - Compare and contrast liquid, solid, and semisolid...Ch. 3.5 - Prob. 23ELOCh. 3.5 - Prob. 24ELOCh. 3.5 - Identify the qualities of enriched, selective, and...Ch. 3.5 - Explain what it means to say that microorganisms...Ch. 3.5 - Describe live media and the circumstances that...Ch. 3.5 - Describe the main purposes of media, and compare...Ch. 3.5 - Differentiate among the ingredients and functions...Ch. 3.5 - Explain the two principal functions of dyes in...Ch. 3.5 - Why are some bacteria difficult to grow in the...Ch. 3.5 - What conditions are necessary to cultivate viruses...Ch. 3.L1 - Prob. 1MCQCh. 3.L1 - Prob. 2MCQCh. 3.L1 - Prob. 3MCQCh. 3.L1 - Prob. 4MCQCh. 3.L1 - Prob. 5MCQCh. 3.L1 - Prob. 6MCQCh. 3.L1 - Resolution is _____________ with a longer...Ch. 3.L1 - Prob. 8MCQCh. 3.L1 - Prob. 9MCQCh. 3.L1 - The specimen for an electron microscope is always...Ch. 3.L1 - Motility is best observed with a a. hanging drop...Ch. 3.L1 - Prob. 12MCQCh. 3.L1 - Prob. 13MCQCh. 3.L1 - Prob. 14MCQCh. 3.L1 - What type of medium is used to maintain and...Ch. 3.L1 - Prob. 16MCQCh. 3.L1 - Multiple Matching. For each type of medium, select...Ch. 3.L1 - Prob. 1CSRCh. 3.L1 - Prob. 2CSRCh. 3.L1 - Prob. 3CSRCh. 3.L1 - Prob. 1WCCh. 3.L1 - Prob. 2WCCh. 3.L1 - Prob. 3WCCh. 3.L1 - Describe the steps of the Gram stain, and explain...Ch. 3.L1 - Describe the steps you would take to isolate,...Ch. 3.L1 - Prob. 6WCCh. 3.L1 - Evaluate the following preparations in terms of...Ch. 3.L2 - A certain medium has the following composition: a....Ch. 3.L2 - a. Name four categories that blood agar fits. b....Ch. 3.L2 - Prob. 3CTCh. 3.L2 - Go back to section 1.2 and observe the six...Ch. 3.L2 - Since the dyes for the Gram stain are not specific...Ch. 3.L2 - Examine figure 3.10a, b (shown here). If you...Ch. 3.L2 - Prob. 2VC
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