Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 46, Problem 1CA
A 45-year-old man was admitted to the hospital with chest and back pain. On examination, his chest was dull to percussion. A chest X-ray film showed lower-left-lung infiltrates. A sputum culture revealed alpha-hemolytic, gram-positive, catalase-negative cocci that were inhibited by optochin. The bacteria produced acid from lactose. Use Appendix H to identify the species of this bacterium.
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List the following to determine the unknown bacteria: Streptococcus mutans
1) Morphology & Characteristics
2) the different biological tests to be done
What is this organism? And what other test could be done to confirm it's identification?
1.Gram stain - positive cocci, chains
Catalase - weak positive
Hemolysis - beta
BE - positive
NaCl- positive
Bacitracin - no zone of inhibition
PYR - positive
Answer: Enterococcus spp. CAMP test to verify the identity
2.Gram positive cocci, chains
Catalase - negative
Hemolysis - alpha
BE - negative
NaCl - negative
P disk - resistant
Bile Solubility - negative
Answer - viridans group, Use PYR test to verify
Why is Salmonella typhi a gram negative bacilli? Explain.
Chapter 46 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
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