Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
expand_more
expand_more
format_list_bulleted
Videos
Question
Chapter 5, Problem 2Q
Summary Introduction
To review:
The result of experiment if protein synthesis of mammalian cell is inhibited at the same time as they were inoculated with the virus carrying gene for fusion protein.
Introduction:
There are many molecules that are able to inhibit protein synthesis in a cell. Antibiotics such as erythromycin and chloramphenicol inhibit protein synthesis. For example, chloramphenicol inhibits peptidyl transfer activity of the ribosome.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Suppose you wanted to study genes controlling thestructure of bacterial cell surfaces. You decide tostart by isolating bacterial mutants resistant to infection by a bacteriophage that binds to the cellsurface. The selection procedure is simple: Spreadcells from a culture of sensitive bacteria on a petriplate, expose them to a high concentration ofphages, and pick the bacterial colonies that grow.To set up the selection you could (1) spread cellsfrom a single liquid culture of sensitive bacteria onmany different plates and pick every resistant colony; or (2) start many different cultures, eachgrown from a single colony of sensitive bacteria,spread one plate from each culture, and then pick asingle mutant from each plate. Which methodwould ensure that you are isolating many independent mutations?
What is reproductive cloning? Are identical twins in humansconsidered to be clones? With regard to agricultural species, whatare some potential advantages to reproductive cloning?
What would happen in the experiment illustrated in Figure if the DNA and RNA that are mixed together came from very different organisms, for example a worm and a pig?
Chapter 5 Solutions
Life: The Science of Biology
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- In two isolates (one is resistant to ampicillin and theother is sensitive to ampicillin) of a new bacterium,you found that genes encoding ampicillin resistanceare being transferred into the sensitive strain. . To determine if the gene transfer involves transformation, conjugation, or transduction, you separatethe ampicillin-resistant and ampicillin-sensitivestrains by a membrane with pores that are smallerthan the size of a bacterium, but larger than thesizes of bacteriophage or DNA fragments. If genetransfer is still observed, what mechanisms arepossibly involved and which are excluded?arrow_forwardA few companies in China and South Korea provide theservice of cloning dogs, using cells from their clients’ petsto provide nuclei in procedures like that in Figure 20.17.Should their clients expect the clone to look identical totheir original pet? Why or why not? What ethical questions does this bring up?arrow_forward. In two isolates (one is resistant to ampicillin and theother is sensitive to ampicillin) of a new bacterium,you found that genes encoding ampicillin resistanceare being transferred into the sensitive strain. . To determine if the gene transfer is transformationor transduction, you treat the mixed culture of cellswith DNase. Why would this treatment distinguishbetween these two modes of gene transfer? Describethe results predicted if the gene transfer is transformation versus transductionarrow_forward
- Researchers who study the molecular mechanism of transformationhave identified many proteins in bacteria that function in theuptake of DNA from the environment and its recombination intothe host cell’s chromosome. This means that bacteria have evolvedmolecular mechanisms for the purpose of transformation by extracellularDNA. What advantage(s) does a bacterium derive from importing DNA from the environment and/or incorporating it into its chromosome?arrow_forwardusing illustrations describe the structure of a typical cloning vector and discuss the functions of each elemnetarrow_forwardWhat would be an advantage of using HaeIII for a cloning experiment? What would be a disadvantage?arrow_forward
- By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the table:Draw a map that shows the order of genes and the locations ofthe origins of transfer among these different Hfr strains?arrow_forwardThe temperature at which the primers and target DNA hybridize may be changed to influence the stringency of PCR amplification. What effect will changing the hybridization temperature have on the amplification? Let's say you have a certain yeast gene A and want to check whether it has a human equivalent. How might managing the hybridization's rigor benefit you?arrow_forwardWhen the cloned cat Carbon Copy (CC) was born , she had black patches and white patches, but completely lacked any orange patches. The knowledgeablestudents of genetics were not surprised at this outcome.Starting with the somatic ovarian cell used as the sourceof the nucleus in the cloning process, explain how this outcomeoccurred.arrow_forward
- Analyse the data proved. Which of the E. coli cultures from the regrowth experiment should be used in mutagenesis experiment. Why you selected and why you rejected？arrow_forwardThe following diagram outlines how the process of cloning a sheep was accomplished. Cloning is the process of creating a genetically identical copy of another individual. With Dolly, the first cloned mammal, an egg cell was removed from a donor (B) and the nucleus was removed from the egg cell. Then cells from a sheep's mammary gland were removed from a second donor (A). The nucleus of one of the cells from the mammary gland was fused with the enucleated egg cell using an electrical pulse. The fused cell underwent cell division and at the blastocyst stage was implanted into a surrogate mother sheep. The fused cell is cultured and is implanted as a multi-celled embryo. During the step where the fused cell begins dividing normally, the cells of the future clone undergo Select one: a. fertilization b. meiosis c. mitosis d. gene splicingarrow_forwardThe following diagram outlines how the process of cloning a sheep was accomplished. Cloning is the process of creating a genetically identical copy of another individual. With Dolly, the first cloned mammal, an egg cell was removed from a donor (B) and the nucleus was removed from the egg cell. Then cells from a sheep's mammary gland were removed from a second donor (A). The nucleus of one of the cells from the mammary gland was fused with the enucleated egg cell using an electrical pulse. The fused cell underwent cell division and at the blastocyst stage was implanted into a surrogate mother sheep. The percentage of genetic material that Dolly (the clone) had in common with Donor A is Select one: a. 25% b. 50% c. 100% d. 0%arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Fossils & Evidence For Evolution | Evolution | Biology | FuseSchool; Author: FuseSchool - Global Education;https://www.youtube.com/watch?v=iYr3sYS9e0w;License: Standard YouTube License, CC-BY
Dig In To Paleontology; Author: SciShow Kids;https://www.youtube.com/watch?v=1FjyKmpmQzc;License: Standard YouTube License, CC-BY