Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 6R
A pastry chef accidentally inoculated a cream pie with six S. aureus cells. If S. aureus has a generation time of 60 minutes, how many cells would be in the cream pie after 7 hours?
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A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109.
a) How long was the generation time in minutes?b) How many generations have occurred?
You inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this organism is 25 minutes. You grew the culture for 10 hours.
a) How many generations occurred?b) How many cells will be present after the 10 hours?
You are doing a viable cell count for a culture of E. coli you are growing in a flask. The volume of media in the flask is 1L. You took an aliquot directly from the media and diluted it using trypan blue. Here are the numbers that you get:
squares counted: 4
total cells counted: 988
total viable cells counted: 960
THESE ARE THE QUESTIONS:
What is your cell viability?
What is the total number of viable cells in your flask?
Show all work.
Given:
Cell Viability = Viable Cells/Total Cells
Cells/mL = Total Viable Cells/Coners Counted x 10,000 x Dilution factor
Chapter 6 Solutions
Microbiology: An Introduction
Ch. 6 - Prob. 1RCh. 6 - Prob. 2RCh. 6 - Prob. 3RCh. 6 - Seven methods of measuring microbial growth were...Ch. 6 - By deep-freezing, bacteria can be stored without...Ch. 6 - A pastry chef accidentally inoculated a cream pie...Ch. 6 - Nitrogen and phosphorus added to beaches following...Ch. 6 - Differentiate complex and chemically defined...Ch. 6 - DRAW IT Draw the following growth curves for E....Ch. 6 - NAME IT A prokaryotic cell hitched a ride to Earth...
Ch. 6 - Two culture media were inoculated with four...Ch. 6 - Two culture media were inoculated with four...Ch. 6 - Use the following graph to answer questions 3 and...Ch. 6 - Use the following graph to answer questions 3 and...Ch. 6 - Assume you inoculated 100 facultatively anaerobic...Ch. 6 - The term trace elements refers to a. the elements...Ch. 6 - Which one of the following temperatures would most...Ch. 6 - Which of the following is not a characteristic of...Ch. 6 - Which of the following types of media would not be...Ch. 6 - An organism that has peroxidase and superoxide...Ch. 6 - E. coli was incubated with aeration in a nutrient...Ch. 6 - Clostridium and Streptococcus are both...Ch. 6 - Most laboratory media contain a fermentable...Ch. 6 - Flask A contains yeast cells in glucose-minimal...Ch. 6 - Clinical Applications and Evaluation 1. Assume...Ch. 6 - Heat lamps are commonly used to maintain foods at...Ch. 6 - The number of bacteria in saliva samples was...
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- If the original number of cells in a sample was 12,000, how many bacteria will be present in 12 hours when the generation time is 15 minutes?arrow_forwardA culture with approximately 3x104 cells/mL were incubated. After 3 hours, the number of cells had increased to 6x105 a) How many generations have occurred?arrow_forwarda. If an egg salad sandwich sitting in a warm car for 4 hours develops 40,960bacterial cells, how many more cells would result in just one more generation? b. What would the cell count be after 4 hours if the initial bacterial dose was 100?c. What do your answers tell you about using clean techniques in food preparation and storage (other than esthetic considerations)?arrow_forward
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- There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one has been incubated for 24 hours. After a 10x dilution by taking 100µl of each culture and adding it to 900 µl water in a microcentrifuge tube and 100µl sample from the tube was taken to view in the counting chamber. a) Count the total number of yeast cells for each culture respectively b) Calculate the concentration and density of yeast cells for each culture respectivelyarrow_forwardAfter treating the bacterium Examplicus expedientius with a chemical agent to test the effectiveness as a disinfectant, you generate a death curve for your bacteria. What is the Decimal Reduction Time for this disinfectant on Examplicus expedientius? Log(10) Cell number 10 9.5 9 8.5 8 7.5 7 6.5 6 5.5 5 0 About 7.5 minutes About 15 minutes About 5 minutes Treatment of Examplicus expedientius Cell Survival About 10 minutes 5 10 15 Time (m) 20 A 30 35arrow_forwardPseudomonas bacteria have porin proteins, are resistant to the chemical triclosan, and survive and can even multiply in quaternary ammonium compounds. True or False?arrow_forward
- In a certain culture of bacteria, the rate of increase is proportional to the number present. (a) if it is found that the number doubles in 6 hours, how many may be expected at the end of 18 hours? (b) If there are 10^2 at the end of 4 hours and 8(10^2) at the end of 8 hours, how many were at the beginning?arrow_forwardWhy is the plate count method a determination of the number of viable cells?arrow_forwardYou are growing a culture of S. aureus. You know that 9 generations have occurred after 6 hours. What is the generation time in minutes? if i started a culture with 1000 cells, after 8 generations, how many cells will i have?arrow_forward
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