The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

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Chapter 7, Problem 1A

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The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

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a. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this sample were plated out, these would theoretically be 10,000 colonies in the Petri plate. Discuss and explain the serial dilutions of this example. b. The disk diffusion method was used to evaluate 3 disinfectants. The results were as follows: Solution Zone of inhibition X 0 mm Y 5 mm Z 10 mm How would you interpret? Provide inference.

A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as 1. diagrammed below. Each dilution tube contained 400 ul of diluent and 100 ul was transferred into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes. a. What is the dilution between each tube shown in the diagram below? Express your answer as a ratio. b. What is the total dilution of tube number 5? Express your answer as a ratio. c. What is the concentration of viable bacteria in the original culture? Express your answer using scientific notation and the units CFU/ml. d. What is the concentration of viable bacteria in tube number 2? Express your answer using the units CFU/ml. e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would you expect to see after the plate was incubated? 1 2 3 5 423 80 13 Number of colonies

A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as 2. diagrammed below. A volume of 500 µl was transferred into each tube. TSA plates were inoculated with 100 µl from the last three dilution tubes. a. If the dilution between each tube is 102, what is the volume of diluent in each of the 5 dilution tubes? Provide the volume using ml as the units. b. What is the total dilution of tube number 4? Express the total dilution using scientific notation. c. What is the concentration of viable bacteria in the original culture? Express the concentration using scientific notation and CFU/ml as the units. d. If you inoculated a TSA plate with 1.0 ml from dilution tube 4, how many colonies would you expect to form on the plate after incubation? e. If the original culture had a volume of 50ml, what was the total number of viable bacteria in the 50 ml of the original culture? 1 2 3…

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Chapter 7, Problem 1A

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The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

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Chapter 7, Problem 1A

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The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

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Chapter 7, Problem 1A

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The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

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Chapter 7, Problem 1A

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Introduction:

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

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Chapter 7, Problem 1A

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The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

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Chapter 7, Problem 1A

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The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

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Ch. 7 - The thermal death time for a suspension of...Ch. 7 - If pasteurization does not achieve sterilization,...Ch. 7 - Thermal death point is not considered an accurate...Ch. 7 - Prob. 4R Ch. 7 - DRAW IT A bacterial culture was in log phase in...Ch. 7 - How do autoclaving, hot air, and pasteurization...Ch. 7 - How do salts and sugars preserve foods? Why are...Ch. 7 - Prob. 8R Ch. 7 - A large hospital washes burn patients in a...Ch. 7 - Prob. 10R

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Solution Summary: The author describes the disk-diffusion method used to evaluate the efficacy of a chemical agent or to test the antibiotic sensitivity of bacteria.

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Introduction:

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

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Chapter 7 Solutions

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Solution Summary: The author describes the disk-diffusion method used to evaluate the efficacy of a chemical agent or to test the antibiotic sensitivity of bacteria.

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Introduction: The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

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Chapter 7 Solutions

Introduction:

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.