Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 8, Problem 5Q
Summary Introduction
To determine:
The reason for not using heat in Kinyoun modification.
Introduction:
The acid-fast stain is used to differentiate between acid-fast bacteria and non-acid fast bacteria. The stain here is used in carbol fuchsin, which is soluble in the lipoidal material found in the mycobacterial cell wall. The decolorizing agent used here is acid alcohol.
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Chapter 8 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 8 - What are the large blue-stained areas on the...Ch. 8 - What is the decolorizing agent in the Gram stain?...Ch. 8 - Prob. 3QCh. 8 - What is phenol (carbolic acid), and what is its...Ch. 8 - Prob. 5QCh. 8 - How might the acid-fast characteristic of...Ch. 8 - In 1882, after experimenting with staining...Ch. 8 - Clinical specimens suspected of containing...Ch. 8 - The acid-fast stain is used to detect...
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- For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain Cationic or Anionic Dye malachite green crystal violet nigrosin safraninarrow_forwardWhy must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?arrow_forwardWhat is the purpose of acid-fast staining? What information can you gain from this procedure? Please explain.arrow_forward
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- Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.arrow_forwardWhat are the principle and basic concepts of Simple staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)arrow_forwardThe above photograph (IMAGE ATTACHED) of a cheek smear was obtained using the high-power objective of a compound microscope. What is the size of the circled cheek cell in millimeters AND nanometers?arrow_forward
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