The map of plasmid pUC19 is shown below. The restriction site coordinate is the position of the 5’base on the top strand of each site sequence. The restriction enzyme sites are in bold type if there is only one site in pUC19. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme PvuII. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme DrdI.
The map of plasmid pUC19 is shown below. The restriction site coordinate is the position of the 5’base on the top strand of each site sequence. The restriction enzyme sites are in bold type if there is only one site in pUC19. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme PvuII. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme DrdI.
Chapter11: Administration Of Nonparenteral Medications
Section: Chapter Questions
Problem 21RQ
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Question
The map of plasmid pUC19 is shown below. The restriction site coordinate is the position of the 5’base on the top strand of each site sequence. The restriction enzyme sites are in bold type if there is only one site in pUC19.
- Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme PvuII.
- Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme DrdI.
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T-Pspl, PI-Scel, Paci, Paek7I, PrlFl,
Pmll, PpuMI, PshAI, Psil, PspOMI,
Sacll, SanDI(x), SexAl, Sfil, SgrAl,
Srfl(x), Stul, Styl, Swal, Tlil,
nl, Xhol
not available from NEB
DrdI 91
BsmBI 51
BSTAPI 179
BsmBI 2683
Ndel 183
Eco01091 2674
KasI - Narl - Sfol 235
AatII - Zral 2617
Вс\VI 2542
SspI 2501
Bgll 245
FspI 256
3.
Pvul 276
Pvull 306
Bmrl 364
AclI 2297
ВсеАl 387
lacza
Apol - EcoRI 396
Banll - SacI - Eco53KI 402
XmnI 2294
Аcс651 - Крnl 408
Aval - BsoBI - Smal -
TspMI - Xmal 412
BcgI 2215
MCS
Scal 2177
BamHI 417
Xbal 423
Pvul 2066
Accl - HinclI - Sall 429
Avall 2059
BspMI - BfuAI 433
Sbfi 434
PUC19
2,686 bp
BsrDI 1935
Pstl 435
AclI 1924
Sphl 441
HindIII 447
FspI 1919
PvulL 628
Avall 1837
Tfil 641
NmeAlII 1822
BglI 1813
Bpml 1784
BsaXI 659
BSPQI - SapI 683
BsrFI 1779
Tfil 781
Bsal 1766
AflIII - Pcil 806
BsrDI 1753
DrdI 908
BmrI 1744
ori
AhdI 1694
BCİVI 1015
BseYI 1110
AlwNI 1217
ВсеАl 1292
第1页,共2页
236 个字 区 英语(美国)
专注
220%
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Transcribed Image Text:自动保存
O Individual Assignment 3
关闭
开始
插入
绘图
设计
布局
引用
邮件
审阅
视图
EndNote X9
图片格式 9告诉我
它共享
口批注
国压缩图片
可组合v
T||高度:
4.4"
更改图片v
删除
背景
艺术效果 透明度
图片边框图片效果
颜色
位置
自动换行 前移一层 后移一层 选择
窗格
对齐
格式
窗格
更正
裁剪
Alt
文本
シ旋转v
宽度:
5.85"
重置图片v
1
2
3
4
7
T-Pspl, PI-Scel, Paci, Paek7I, PrlFl,
Pmll, PpuMI, PshAI, Psil, PspOMI,
Sacll, SanDI(x), SexAl, Sfil, SgrAl,
Srfl(x), Stul, Styl, Swal, Tlil,
nl, Xhol
not available from NEB
DrdI 91
BsmBI 51
BSTAPI 179
BsmBI 2683
Ndel 183
Eco01091 2674
KasI - Narl - Sfol 235
AatII - Zral 2617
Вс\VI 2542
SspI 2501
Bgll 245
FspI 256
3.
Pvul 276
Pvull 306
Bmrl 364
AclI 2297
ВсеАl 387
lacza
Apol - EcoRI 396
Banll - SacI - Eco53KI 402
XmnI 2294
Аcс651 - Крnl 408
Aval - BsoBI - Smal -
TspMI - Xmal 412
BcgI 2215
MCS
Scal 2177
BamHI 417
Xbal 423
Pvul 2066
Accl - HinclI - Sall 429
Avall 2059
BspMI - BfuAI 433
Sbfi 434
PUC19
2,686 bp
BsrDI 1935
Pstl 435
AclI 1924
Sphl 441
HindIII 447
FspI 1919
PvulL 628
Avall 1837
Tfil 641
NmeAlII 1822
BglI 1813
Bpml 1784
BsaXI 659
BSPQI - SapI 683
BsrFI 1779
Tfil 781
Bsal 1766
AflIII - Pcil 806
BsrDI 1753
DrdI 908
BmrI 1744
ori
AhdI 1694
BCİVI 1015
BseYI 1110
AlwNI 1217
ВсеАl 1292
第1页,共2页
236 个字 区 英语(美国)
专注
220%
+
< >
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